Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells.
Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells.
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DOI：
10.3390/ma9040283
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发表时间：
2016-04-13
期刊：
影响因子：
--
通讯作者：
Ishikawa K
中科院分区：
文献类型：
--
作者：
Takechi M;Ninomiya Y;Ohta K;Tada M;Sasaki K;Rahman MZ;Ohta A;Tsuru K;Ishikawa K
为了提高磷灰石水泥（AC）的骨传导性以重建口腔颌面手术后的骨缺损，我们之前制备了含有去端肽胶原（AC（ate））的AC。在本研究中，我们检查了小鼠成骨细胞（MC3T3-E1细胞）在传统AC（c-AC）、AC（ate）和塑料细胞皿表面上的初始附着、增殖和分化。显示出最初附着于AC(ate)的成骨细胞数量大于附着于c-AC的成骨细胞数量，并且与附着于塑料细胞孔的数量相似。我们还发现，与 c-AC 和没有标本的孔相比，AC(ate) 上的成骨细胞铺展良好，数量增加，而 AC(ate) 三天后，成骨细胞中产生的 I 型前胶原羧基端肽 (PIPC) 量比其他细胞更多。三天和六天后，三种表面类型的成骨细胞的碱性磷酸酶（ALP）活性和骨钙素产生没有显着差异。然而，12 天后，AC(ate) 组的 ALP 活性和产生的骨钙素更高。总之，AC(ate)可能是一种具有高骨传导性的材料，可用于口腔颌面术后骨缺损的重建。
To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.
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影响因子：
14
作者：
Miyamato, Y;Ishikawa, K;Asaoka, K
通讯作者：
Asaoka, K
影响因子：
14
作者：
MIYAMOTO, Y;ISHIKAWA, K;ASAOKA, K
通讯作者：
ASAOKA, K
影响因子：
1.9
作者：
KENT, JN;QUINN, JH;BOYNE, PJ
通讯作者：
BOYNE, PJ
影响因子：
3.7
作者：
LYNCH, MP;STEIN, JL;LIAN, JB
通讯作者：
LIAN, JB
影响因子：
4.2
作者：
GILLES, JA;CARNES, DL;WINDELER, AS
通讯作者：
WINDELER, AS