Voluntary exercise can strengthen the circadian system in aged mice.
Voluntary exercise can strengthen the circadian system in aged mice.
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DOI：
10.1007/s11357-012-9502-y
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Biello, S. M.
中科院分区：
文献类型：
--
作者：
Leise, T. L.;Harrington, M. E.;Molyneux, P. C.;Song, I.;Queenan, H.;Zimmerman, E.;Lall, G. S.;Biello, S. M.
根据一项将昼夜节律紊乱与负面健康影响联系起来的研究，一致的日常节律对健康衰老很重要。我们研究了年龄和锻炼对基线昼夜节律的影响，以及作为对系统稳健性的测试，昼夜节律系统对由光：暗(LD)周期提前8小时引起的扰动的反应能力。小鼠(雄性，mPer2luc/C57BL/6)在两个日龄之一进行研究：3.5月龄(n = 39)和18月龄(n = 72)。我们检查了这些小鼠在束缚和移动条件下的活动记录，以及mPER2：：Luc体外测量，以评估视交叉上核(SCN)和重要靶器官的昼夜节律功能。年龄与减少转轮的使用，活动的碎片化，以及在行为和分子测量中减慢重置有关。此外，我们观察到，对于老年小鼠，在体外SCN中，滚轮的存在改变了自发放电频率节律的幅度。随着LD周期的改变，年轻和老年小鼠的体外SCN的mPER2：：Luc振荡的节律性都发生了变化，老年小鼠表现出更持久的内部去同步化。接触到跑轮可以缓解生理系统中与年龄相关的一些变化。在另一项实验中，我们复制了转轮的作用，比较了有或没有转轮的老年小鼠的行为和体外结果(21个月，每组n = 8，都在换班后4天进行了检查)。自愿运动对老年动物昼夜节律特性的影响是一个新的发现，对生活在环境导致的昼夜节律紊乱的老年人的健康有影响。本文的在线版本(doi：10.1007/s11357-0129502-y)包含补充材料，授权用户可以使用。
Consistent daily rhythms are important to healthy aging according to studies linking disrupted circadian rhythms with negative health impacts. We studied the effects of age and exercise on baseline circadian rhythms and on the circadian system's ability to respond to the perturbation induced by an 8 h advance of the light:dark (LD) cycle as a test of the system's robustness. Mice (male, mPer2luc/C57BL/6) were studied at one of two ages: 3.5 months (n = 39) and >18 months (n = 72). We examined activity records of these mice under entrained and shifted conditions as well as mPER2::LUC measures ex vivo to assess circadian function in the suprachiasmatic nuclei (SCN) and important target organs. Age was associated with reduced running wheel use, fragmentation of activity, and slowed resetting in both behavioral and molecular measures. Furthermore, we observed that for aged mice, the presence of a running wheel altered the amplitude of the spontaneous firing rate rhythm in the SCN in vitro. Following a shift of the LD cycle, both young and aged mice showed a change in rhythmicity properties of the mPER2::LUC oscillation of the SCN in vitro, and aged mice exhibited longer lasting internal desynchrony. Access to a running wheel alleviated some age-related changes in the circadian system. In an additional experiment, we replicated the effect of the running wheel, comparing behavioral and in vitro results from aged mice housed with or without a running wheel (>21 months, n = 8 per group, all examined 4 days after the shift). The impact of voluntary exercise on circadian rhythm properties in an aged animal is a novel finding and has implications for the health of older people living with environmentally induced circadian disruption. The online version of this article (doi:10.1007/s11357-012-9502-y) contains supplementary material, which is available to authorized users.
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影响因子：
5.6
作者：
Buysse, DJ;Monk, TH;Begley, A
通讯作者：
Begley, A
影响因子：
3.4
作者：
Duncan, Marilyn J.;Hester, James M.;Franklin, Kathleen M.
通讯作者：
Franklin, Kathleen M.
影响因子：
25
作者：
Deboer, T;Vansteensel, MJ;Meijer, JH
通讯作者：
Meijer, JH
影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.4049/jimmunol.1001026
发表时间：
2010-11-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Castanon-Cervantes O;Wu M;Ehlen JC;Paul K;Gamble KL;Johnson RL;Besing RC;Menaker M;Gewirtz AT;Davidson AJ
通讯作者：
Davidson AJ