Perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) and DNA methylation in newborn dried blood spots in the Upstate KIDS cohort.
Perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) and DNA methylation in newborn dried blood spots in the Upstate KIDS cohort.
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全氟辛酸(PFOA)或全氟辛烷磺酸(PFOS)与北部儿童队列中新生儿干血斑点的DNA甲基化。
DOI：
10.1016/j.envres.2020.110668
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发表时间：
2021-03
影响因子：
8.3
通讯作者：
Yeung EH
中科院分区：
文献类型：
--
作者：
Robinson SL;Zeng X;Guan W;Sundaram R;Mendola P;Putnick DL;Waterland RA;Gunasekara CJ;Kannan K;Gao C;Bell EM;Yeung EH
全氟辛酸(PFOA)和全氟辛烷磺酸(PFOS)是持久性有机污染物，可能通过表观遗传修饰改变产前发育。先前研究全氟辛烷磺酸/全氟辛烷磺酸和DNA甲基化的受试者相对较少(n<；200)，且结果不一致。在北部儿童队列研究中，我们检测了597名新生儿的全氟辛烷磺酸/全氟辛烷磺酸与DNA甲基化的关系。用高效液相色谱/串联质谱仪测定新生儿血样中全氟辛烷磺酸/全氟辛烷磺酸的含量。从DBS中提取DNA，用Infinium甲基化EPIC珠芯片检测DNA甲基化。用稳健的线性回归分析了全氟辛烷磺酸/全氟辛烷磺酸与单个CpG位点DNA甲基化的关系。协变量包括样本板、估计的细胞类型、表观遗传起源的祖先、婴儿性别和多发性、母亲社会经济地位的指标以及先前的妊娠损失。在补充分析中，我们将分析限制在2242个先前被确定为系统个体间变异相关区域(CoRSIV)的CpG位点，其中包括亚稳态表位基因。在FDR<；0.05，全氟辛酸浓度和第90个百分位数与位于SCRT2附近的cg15557840的DNA甲基化有关，SRXN1；pFOS>；90%与2个CpG位点的性别相关(男孩的cg19039925和女孩的ZNF26的cg05754408)。当分析限于CoRSIV时，对数标度的、连续的全氟辛烷磺酸浓度与PTBP1内cg03278866的DNA甲基化有关。总而言之，在北部儿童队列中，新生儿DBS中高浓度的全氟辛烷磺酸/全氟辛烷磺酸与DNA甲基化之间存在关联的证据有限。这些发现值得在全氟辛烷磺酸/全氟辛烷磺酸中值浓度较高的人群中推广。
Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are persistent organic pollutants which may alter prenatal development, potentially through epigenetic modifications. Prior studies examining PFOS/PFOA and DNA methylation have relatively few subjects (n<200) and inconsistent results. We examined relations of PFOA/PFOS with DNA methylation among 597 neonates in the Upstate KIDS cohort study. PFOA/PFOS were quantified in newborn dried blood spots (DBS) using high-performance liquid chromatography/tandem mass spectrometry. DNA methylation was measured using the Infinium MethylationEPIC BeadChip with DNA extracted from DBS. Robust linear regression was used to examine the associations of PFOA/PFOS with DNA methylation at individual CpG sites. Covariates included sample plate, estimated cell type, epigenetically derived ancestry, infant sex and plurality, indicators of maternal socioeconomic status, and prior pregnancy loss. In supplemental analysis, we restricted the analysis to 2242 CpG sites previously identified as Correlated Regions of Systemic Interindividual Variation (CoRSIVs) which include metastable epialleles. At FDR<0.05, PFOA concentration >90th percentile was related to DNA methylation at cg15557840, near SCRT2, SRXN1; PFOS>90th percentile was related to 2 CpG sites in a sex-specific manner (cg19039925 in GVIN1 in boys and cg05754408 in ZNF26 in girls). When analysis was restricted to CoRSIVs, log-scaled, continuous PFOS concentration was related to DNA methylation at cg03278866 within PTBP1. In conclusion, there was limited evidence of an association between high concentrations of PFOA/PFOS and DNA methylation in newborn DBS in the Upstate KIDS cohort. These findings merit replication in populations with a higher median concentration of PFOA/PFOS.
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DOI：
10.3390/ijerph15061066
发表时间：
2018-05-24
影响因子：
--
作者：
Liu CY;Chen PC;Lien PC;Liao YP
通讯作者：
Liao YP
影响因子：
3.7
作者：
Guerrero-Preston, Rafael;Goldman, Lynn R.;Sidransky, David
通讯作者：
Sidransky, David
影响因子：
6.2
作者：
Kato, Kayoko;Wanigatunga, Amal A.;Calafat, Antonia M.
通讯作者：
Calafat, Antonia M.
影响因子：
5.7
作者：
Ouidir, Marion;Mendola, Pauline;Tekola-Ayele, Fasil
通讯作者：
Tekola-Ayele, Fasil
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y