High throughput phenotyping for aphid resistance in large plant collections.
High throughput phenotyping for aphid resistance in large plant collections.
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DOI：
10.1186/1746-4811-8-33
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发表时间：
2012-08-17
期刊：
影响因子：
5.1
通讯作者：
Broekgaarden C
中科院分区：
文献类型：
--
作者：
Chen X;Vosman B;Visser RG;van der Vlugt RA;Broekgaarden C
以韧皮部为食的昆虫是世界上最具破坏性的害虫之一。它们不仅通过取食韧皮部造成损害，从而耗尽植物的光同化物，而且还通过传播病毒。到目前为止，防止这类问题的主要方法是经常使用杀虫剂。应用抗性品种将是更环保和可持续的解决方案。为此，需要首先确定耐药来源。目前还没有适合的高通量表型分析方法来鉴定植物对韧皮部食用虫的抗性来源。在本文中，我们提出了一种高通量筛选系统，以鉴定对蚜虫抗性增强的植物。它的多功能性是通过拟南芥激活标签突变系收集来证明的。该系统由青桃蚜（Myzus persicae, Sulzer）和芜菁黄病毒（Turnip yellow virus, TuYV）组成。在初步筛选中，一株植物代表一个突变系，通过ELISA鉴定出13个无病毒突变系。利用这些品系的种子，对候选品系进行了重新评价和鉴定，获得了9个对蚜虫抗性增强的品系。这种桃蚜- tuyv筛选系统是一种高效、可靠和快速的方法，可从数千个突变品系中鉴定出抗蚜品系。在我们的研究中，一个人在短短5个月内从5160个突变品系中筛选出9个抗蚜性增强的突变品系。该系统可扩展到其他韧皮部取食昆虫和循环病毒，以从若干收集物中识别抗虫源，例如包括基因库和人工制备的突变体收集物。
Phloem-feeding insects are among the most devastating pests worldwide. They not only cause damage by feeding from the phloem, thereby depleting the plant from photo-assimilates, but also by vectoring viruses. Until now, the main way to prevent such problems is the frequent use of insecticides. Applying resistant varieties would be a more environmental friendly and sustainable solution. For this, resistant sources need to be identified first. Up to now there were no methods suitable for high throughput phenotyping of plant germplasm to identify sources of resistance towards phloem-feeding insects. In this paper we present a high throughput screening system to identify plants with an increased resistance against aphids. Its versatility is demonstrated using an Arabidopsis thaliana activation tag mutant line collection. This system consists of the green peach aphid Myzus persicae (Sulzer) and the circulative virus Turnip yellows virus (TuYV). In an initial screening, with one plant representing one mutant line, 13 virus-free mutant lines were identified by ELISA. Using seeds produced from these lines, the putative candidates were re-evaluated and characterized, resulting in nine lines with increased resistance towards the aphid. This M. persicae-TuYV screening system is an efficient, reliable and quick procedure to identify among thousands of mutated lines those resistant to aphids. In our study, nine mutant lines with increased resistance against the aphid were selected among 5160 mutant lines in just 5 months by one person. The system can be extended to other phloem-feeding insects and circulative viruses to identify insect resistant sources from several collections, including for example genebanks and artificially prepared mutant collections.
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影响因子：
4.5
作者：
Murphy, JF;Zehnder, GW;Kloepper, JW
通讯作者：
Kloepper, JW
DOI：
10.1099/00221287-25-3-459
发表时间：
1961-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
KASSANIS, B;NIXON, HL
通讯作者：
NIXON, HL
影响因子：
7.4
作者：
Marsch-Martinez, N;Greco, R;Pereira, A
通讯作者：
Pereira, A
影响因子：
1.9
作者：
Alvarez, A. E.;Garzo, E.;Tjallingii, W. F.
通讯作者：
Tjallingii, W. F.
影响因子：
7.4
作者：
Mewis, I;Appel, HM;Schultz, JC
通讯作者：
Schultz, JC