Damages to the extracellular matrix in articular cartilage due to cryopreservation by microscopic magnetic resonance imaging and biochemistry.
Damages to the extracellular matrix in articular cartilage due to cryopreservation by microscopic magnetic resonance imaging and biochemistry.
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由于微观磁共振成像和生物化学而导致关节软骨的细胞外基质的损害。
DOI：
10.1016/j.mri.2008.10.003
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发表时间：
2009-06
影响因子：
2.5
通讯作者：
Duvoisin, Nick
中科院分区：
文献类型：
--
作者：
Zheng, ShaoKuan;Xia, Yang;Bidthanapally, Aruna;Badar, Farid;Ilsar, Itamar;Duvoisin, Nick
关键词：
为探讨冷冻保存对关节软骨细胞外基质的损伤作用，采用DGEMRIC体外显微核磁共振成像技术(μMRI)，对34例犬肩关节软骨标本进行了13μm像素分辨率的糖胺多聚糖(GAG)浓度随深度变化的成像。此外，生化分析被用来确定从组织到组织浸泡的溶液中的GAG损失。在没有任何冷冻保护剂的情况下，在−20°C或−80°C冷冻的标本中，软骨中的GAG显著丢失(高达56.5%)，这取决于组织的结构区和冷冻保存条件。研究了二甲基亚砜(DMSO)的低温保护能力与其在生理盐水中的浓度和储存温度的关系。在−20℃的保存温度下，30%的二甲基亚砜浓度足以防止组织中GAG的减少，但−80℃的超低温保存需要50%的二甲基亚砜浓度。这些成像结果通过生化分析得到了验证。
To investigate the damages to the extracellular matrix in articular cartilage due to cryopreservation, the depth-dependent concentration profiles of glycosaminoglycans (GAGs) in thirty-four cartilage specimens from canine humeral heads were imaged at 13μm pixel resolution using the in vitro version of the dGEMRIC protocol in microscopic MRI (μMRI). In addition, a biochemical assay was used to determine the GAG loss from the tissue to the solution where the tissue was immersed. For specimens that had been frozen at −20 °C or −80 °C without any cryoprotectant, a significant loss of GAG (as high as 56.5%) was found in cartilage, dependent upon the structural zones of the tissue and the conditions of cryopreservation. The cryoprotective abilities of dimethyl sulfoxide (DMSO) as a function of its concentration in saline and storage temperature were also investigated. A 30% DMSO concentration was sufficient in preventing the reduction of GAG in the tissue at the −20 °C storage temperature, but a 50% concentration of DMSO was necessary for the −80 °C cryopreservation. These imaging results were verified by the biochemical analysis.
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