Inorganic Nitrogen Application Affects Both Taxonomical and Predicted Functional Structure of Wheat Rhizosphere Bacterial Communities.
Inorganic Nitrogen Application Affects Both Taxonomical and Predicted Functional Structure of Wheat Rhizosphere Bacterial Communities.
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DOI：
10.3389/fmicb.2018.01074
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发表时间：
2018
影响因子：
5.2
通讯作者：
Mauchline TH
中科院分区：
文献类型：
--
作者：
Kavamura VN;Hayat R;Clark IM;Rossmann M;Mendes R;Hirsch PR;Mauchline TH
施肥制度对大量土壤微生物群落的影响已经得到了很好的研究，但这不是根际微生物组的情况。这项工作的目的是评估施肥制度对小麦根际微生物组组装和16S rRNA基因预测的功能与土壤的长期Broadbalk实验在Rothamsted研究的影响。在有机氮、无氮、中无机氮和高无机氮4种施氮水平下，用普通小麦（Triticum aestivum cv.）华彩乐段用Illumina平台对4周龄和开花植物（10周）的大量土壤和根际样品进行16S rRNA基因扩增子测序。进行系统发育和16S rRNA基因预测功能分析。施肥制度影响小麦根际细菌群落的结构和组成。酸杆菌和浮游菌显着耗尽在接受无机N的治疗，而除了高水平的无机N丰富的成员的拟杆菌门，特别是在10周后。细菌丰富度和多样性随着无机氮的输入而降低，有机处理（FYM）后最高。一般来说，高水平的无机氮肥会对细菌的丰富度和多样性产生负面影响，导致细菌群落结构随着时间的推移而变得不稳定，而有机改良剂则提供了更稳定的细菌群落。16S rRNA基因预测的功能结构受生长阶段的影响大于施肥处理，虽然，一些功能相关的能量代谢和代谢的萜类化合物和聚酮化合物富集在样品不接受任何无机氮，而无机氮添加丰富预测功能相关的其他氨基酸和碳水化合物的代谢。了解不同肥料对根际微生物组结构和动态的影响是制定可持续作物生产战略的重要一步。
The effects of fertilizer regime on bulk soil microbial communities have been well studied, but this is not the case for the rhizosphere microbiome. The aim of this work was to assess the impact of fertilization regime on wheat rhizosphere microbiome assembly and 16S rRNA gene-predicted functions with soil from the long term Broadbalk experiment at Rothamsted Research. Soil from four N fertilization regimes (organic N, zero N, medium inorganic N and high inorganic N) was sown with seeds of Triticum aestivum cv. Cadenza. 16S rRNA gene amplicon sequencing was performed with the Illumina platform on bulk soil and rhizosphere samples of 4-week-old and flowering plants (10 weeks). Phylogenetic and 16S rRNA gene-predicted functional analyses were performed. Fertilization regime affected the structure and composition of wheat rhizosphere bacterial communities. Acidobacteria and Planctomycetes were significantly depleted in treatments receiving inorganic N, whereas the addition of high levels of inorganic N enriched members of the phylum Bacteroidetes, especially after 10 weeks. Bacterial richness and diversity decreased with inorganic nitrogen inputs and was highest after organic treatment (FYM). In general, high levels of inorganic nitrogen fertilizers negatively affect bacterial richness and diversity, leading to a less stable bacterial community structure over time, whereas, more stable bacterial communities are provided by organic amendments. 16S rRNA gene-predicted functional structure was more affected by growth stage than by fertilizer treatment, although, some functions related to energy metabolism and metabolism of terpenoids and polyketides were enriched in samples not receiving any inorganic N, whereas inorganic N addition enriched predicted functions related to metabolism of other amino acids and carbohydrates. Understanding the impact of different fertilizers on the structure and dynamics of the rhizosphere microbiome is an important step toward developing strategies for production of crops in a sustainable way.
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影响因子：
9.7
作者：
Clark, Ian M.;Hirsch, Penny R.
通讯作者：
Hirsch, Penny R.
影响因子：
2.7
作者：
Basu, Supratim;Rabara, Roel C.;Negi, Sangeeta
通讯作者：
Negi, Sangeeta
影响因子：
4.8
作者：
Fierer, Noah;Bradford, Mark A.;Jackson, Robert B.
通讯作者：
Jackson, Robert B.
35年无机肥及粪肥改良对东北黑土细菌和古菌群落结构的影响
影响因子：
4.8
作者：
Ding, Jianli;Jiang, Xin;Li, Jun
通讯作者：
Li, Jun
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y