Exogenous miRNAs induce post-transcriptional gene silencing in plants.
Exogenous miRNAs induce post-transcriptional gene silencing in plants.
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DOI：
10.1038/s41477-021-01005-w
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发表时间：
2021-10
期刊：
影响因子：
18
通讯作者：
Perata P
中科院分区：
文献类型：
--
作者：
Betti F;Ladera-Carmona MJ;Weits DA;Ferri G;Iacopino S;Novi G;Svezia B;Kunkowska AB;Santaniello A;Piaggesi A;Loreti E;Perata P
植物似乎吸收了人为设计的靶向特定基因的外源RNA，随后激活了RNA干扰（RNAi）机制。然而，目前还不知道植物是否使用RNA本身作为植物与植物之间通信的信号分子，除了证据表明寄生植物与其宿主之间发生小RNA的交换。来自环境的外源RNA，如果被某些生物体吸收，确实可以诱导RNAi。这种现象已经在线虫和昆虫中观察到，并且宿主拟南芥细胞分泌外泌体样细胞外囊泡以将植物小RNA递送到灰葡萄孢中。在这里，我们表明，由植物产生的micro-RNAs（miRNAs）作为信号分子影响其他附近植物的基因表达。外源性miRNA（例如miR 156和miR 399）通过需要AGO 1和RDR 6的机制触发RNAi。这强调了次级小干扰RNA的产生是必需的。这一证据强调了一种机制的存在，其中miRNA代表了使植物之间能够进行通信的信号分子。这项研究表明，植物产生的miRNAs作为信号分子影响附近植物的基因表达。这种由外源miRNA诱导的RNAi使得植物之间能够进行通信，并且需要产生次级siRNA。
Plants seem to take up exogenous RNA that was artificially designed to target specific genes, followed by activation of the RNA interference (RNAi) machinery. It is, however, not known whether plants use RNAs themselves as signalling molecules in plant-to-plant communication, other than evidence that an exchange of small RNAs occurs between parasitic plants and their hosts. Exogenous RNAs from the environment, if taken up by some living organisms, can indeed induce RNAi. This phenomenon has been observed in nematodes and insects, and host Arabidopsis cells secrete exosome-like extracellular vesicles to deliver plant small RNAs into Botrytis cinerea. Here we show that micro-RNAs (miRNAs) produced by plants act as signalling molecules affecting gene expression in other, nearby plants. Exogenous miRNAs, such as miR156 and miR399, trigger RNAi via a mechanism requiring both AGO1 and RDR6. This emphasizes that the production of secondary small interfering RNAs is required. This evidence highlights the existence of a mechanism in which miRNAs represent signalling molecules that enable communication between plants. This study shows that miRNAs produced by plants act as signalling molecules that affect gene expression in nearby plants. This RNAi induced by exogenous miRNAs enables communication between plants and requires the production of secondary siRNAs.
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影响因子：
7.2
作者：
Pant, Bikram Datt;Buhtz, Anja;Kehr, Julia;Scheible, Wolf-Ruediger
通讯作者：
Scheible, Wolf-Ruediger
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
7.4
作者：
Iacopino, Sergio;Jurinovich, Sandro;Licausi, Francesco
通讯作者：
Licausi, Francesco
跨王国RNA贩运和环境RNAi-Nature的蓝图，用于现代作物保护策略。
影响因子：
5.4
作者：
Cai Q;He B;Kogel KH;Jin H
通讯作者：
Jin H
DOI：
10.1038/nrm4085
发表时间：
2015-12
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Borges F;Martienssen RA
通讯作者：
Martienssen RA