Membrane mixing and dynamics in hybrid POPC/poly(1,2-butadiene-block-ethylene oxide) (PBd-b-PEO) lipid/block co-polymer giant vesicles.
Membrane mixing and dynamics in hybrid POPC/poly(1,2-butadiene-block-ethylene oxide) (PBd-b-PEO) lipid/block co-polymer giant vesicles.
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杂化 POPC/聚（1,2-丁二烯-嵌段-环氧乙烷）（PBd-b-PEO）脂质/嵌段共聚物巨型囊泡中的膜混合和动力学。
DOI：
10.1039/d1sm01591e
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发表时间：
2022
期刊：
影响因子：
3.4
通讯作者：
Seneviratne R
中科院分区：
文献类型：
--
作者：
Seneviratne R
脂质和嵌段共聚物可以单独地自组装成囊泡，每种都具有它们自己的特定益处和限制。将聚合物与脂质组合允许进一步优化用于生物纳米技术应用的囊泡膜。在此，将POPC脂质与两种不同分子量的聚（1，2-丁二烯嵌段-环氧乙烷）（PBd 22-PEO 14，Mr = 1800 g mol−1和PBd 12-PEO 11，Mr = 1150 g mol−1）混合，以研究增加聚合物分数如何影响膜混合、水合和流动性。混合GUV膜内荧光标记的脂质和聚合物的强度贡献证实了混合物内的膜均匀性。在GUV中的Laurdan的一般极化测量显示，随着聚合物分数的增加，膜水合几乎没有变化，这表明脂质和聚合物之间具有良好的结构相容性，从而产生良好混合的囊泡。发现混合GUV中的膜流动性随着聚合物分数的增加而非线性地降低。然而，在杂化膜中的荧光聚合物的扩散系数并没有随着聚合物含量的增加而显著改变。虽然增加聚合物分数确实减少了脂质通过富含聚合物的基质的运动，但聚合物的扩散系数的差异并不显著，这表明在研究的范围内，其扩散受脂质组合物增加的影响最小。这些结果奠定了进一步的基础，更广泛的发展与先进的生物技术控制性能的混合囊泡。
Lipids and block copolymers can individually self-assemble into vesicles, each with their own particular benefits and limitations. Combining polymers with lipids allows for further optimisation of the vesicle membranes for bionanotechnology applications. Here, POPC lipid is mixed with poly(1,2-butadiene-block-ethylene oxide) of two different molecular weights (PBd22–PEO14, Mr = 1800 g mol−1 and PBd12–PEO11, Mr = 1150 g mol−1) in order to investigate how increasing the polymer fraction affects membrane mixing, hydration and fluidity. Intensity contributions of fluorescently labelled lipid and polymer within mixed GUV membranes confirm membrane homogeneity within the hybrids. General polarisation measurements of Laurdan in GUVs showed little change in membrane hydration as polymer fraction is increased, which suggests good structural compatibility between lipids and polymers that gives rise to well-mixed vesicles. Membrane fluidity in hybrid GUVs was found to decrease non-linearly with increasing polymer fraction. However, the diffusion coefficients for the fluorescent polymer in hybrid membranes did not change significantly with increasing polymer content. While increasing the polymer fraction does reduce the movement of lipids through a polymer-rich matrix, insignificant difference in diffusion coefficients of the polymer suggests that its diffusion is minimally affected by increasing lipid composition in the range studied. These results lay further foundations for the wider development of hybrid vesicles with controlled properties for advanced biotechnologies.
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影响因子：
4.7
作者：
Cheng, Zhiliang;Elias, Drew R.;Kamat, Neha P.;Johnston, Eric D.;Poloukhtine, Andrei;Popik, Vladimir;Hammer, Daniel A.;Tsourkas, Andrew
通讯作者：
Tsourkas, Andrew
影响因子：
3.2
作者：
Kleineberg, Christin;Woelfer, Christian;Vidakovic-Koch, Tanja
通讯作者：
Vidakovic-Koch, Tanja
DOI：
10.1002/cphc.201402794
发表时间：
2015-05-18
期刊：
Chemphyschem : a European journal of chemical physics and physical chemistry
影响因子：
--
作者：
Sezgin E;Waithe D;Bernardino de la Serna J;Eggeling C
通讯作者：
Eggeling C
监测和调节混合脂质/聚合物囊泡中的离子流量。
DOI：
--
发表时间：
2017
期刊：
Colloids and Surfaces B: Biointerfaces
影响因子：
--
作者：
W. Paxton;Patrick T McAninch;K. Achyuthan;S. Shin;Haley L Monteith
通讯作者：
Haley L Monteith