Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.
Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.
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DOI：
10.1021/bc200214g
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发表时间：
2011-10-19
影响因子：
4.7
通讯作者：
Tsourkas, Andrew
中科院分区：
文献类型：
--
作者：
Cheng, Zhiliang;Elias, Drew R.;Kamat, Neha P.;Johnston, Eric D.;Poloukhtine, Andrei;Popik, Vladimir;Hammer, Daniel A.;Tsourkas, Andrew
基于嵌段共聚物的囊泡由于其独特的结构特性，最近作为药物递送和分子成像应用的纳米平台获得了极大的兴趣。这些纳米囊泡已被证明可以通过增强的渗透性和保留性或甚至更有效地通过主动靶向将其货物引导到疾病部位。在这里，我们表明，与单独的嵌段共聚物相比，从聚合物-脂质共混物制备纳米囊泡靶向的功效可以显着提高。聚合物-脂质杂化纳米囊泡由二嵌段共聚物，聚（环氧乙烷）-嵌段-聚丁二烯（PEO-PBD）和磷脂，氢化大豆磷脂酰胆碱（HSPC）的水性共组装产生。基于PEG的囊泡，直径117 nm，用叶酸或抗HER 2/neu抗体作为靶向配体进行功能化，以赋予癌细胞特异性。我们的结果显示，在体外细胞研究和鼠肿瘤模型中，与单独由嵌段共聚物制备的纳米囊泡相比，由聚合物-脂质共混物制备的纳米囊泡导致细胞结合的显著改善。因此，可以预见，由聚合物-脂质混合物组成的纳米囊泡可以构成靶向药物递送和分子成像应用的优选实施方案。
Block copolymer-based vesicles have recently garnered a great deal of interest as nanoplatforms for drug delivery and molecular imaging applications due to their unique structural properties. These nanovesicles have been shown to direct their cargo to disease sites either through enhanced permeability and retention or even more efficiently via active targeting. Here we show that the efficacy of nanovesicle targeting can be significantly improved when prepared from polymer-lipid blends compared with block copolymer alone. Polymer-lipid hybrid nanovesicles were produced from the aqueous co-assembly of the diblock copolymer, poly(ethylene oxide)-block-polybutadiene (PEO-PBD), and the phospholipid, hydrogenated soy phosphatidylcholine (HSPC). The PEG-based vesicles, 117 nm in diameter, were functionalized with either folic acid or anti-HER2/neu affibodies as targeting ligands to confer specificity for cancer cells. Our results revealed that nanovesicles prepared from polymer-lipid blends led to significant improvement in cell binding compared to nanovesicles prepared from block copolymer alone in both in vitro cell studies and murine tumor models. Therefore, it is envisioned that nanovesicles composed of polymer-lipid blends may constitute a preferred embodiment for targeted drug delivery and molecular imaging applications.
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影响因子：
1.8
作者：
Le Meins, J. -F.;Sandre, O.;Lecommandoux, S.
通讯作者：
Lecommandoux, S.
影响因子：
3.4
作者：
HEERKLOTZ, H;BINDER, H;KLOSE, G
通讯作者：
KLOSE, G
影响因子：
1.5
作者：
Liu, Yang;Sun, Jin;He, Zhonggui
通讯作者：
He, Zhonggui
影响因子：
3.9
作者：
Pangu, Gautani;Johnston, Eric;Hammer, Daniel A.
通讯作者：
Hammer, Daniel A.
影响因子：
4.7
作者：
Gabizon, A;Horowitz, AT;Zalipsky, S
通讯作者：
Zalipsky, S