β2‐Adrenergic stimulation causes detachment of natural killer cells from cultured endothelium
β2‐Adrenergic stimulation causes detachment of natural killer cells from cultured endothelium
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β2-肾上腺素能刺激导致自然杀伤细胞与培养的内皮细胞分离
DOI：
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发表时间：
1993
影响因子：
5.4
通讯作者：
R. Ballieux
中科院分区：
文献类型：
--
作者：
R. Benschop;F. Oostveen;C. Heijnen;R. Ballieux
体育锻炼、精神压力或β -肾上腺素能激动剂的输注可导致外周循环中自然杀伤（NK）细胞数量的增加。鉴于NK细胞在体内的特定迁移模式，有人认为这些细胞可能是从血管中的边缘池中释放出来的。在本报告中，表征了儿茶酚胺对NK细胞与未刺激的人内皮细胞（EC）粘附的体外影响。将外周血单核细胞粘附在EC单层上，然后用流式细胞术分析粘附淋巴细胞的表型。NK细胞被发现优先粘附于EC，这一过程被添加各种肾上腺素能激动剂逆转。儿茶酚胺选择性地影响NK细胞的粘附，而对T细胞对EC的粘附没有影响，正如使用纯化细胞群所确定的那样。与肾上腺素（EPI）短时间孵育（5分钟）可使NK细胞从EC中分离出来，并且与浓度相关，ED50为2 × 10−10M。通过α和β -肾上腺素能激动剂和拮抗剂，我们发现NK细胞的脱离是通过β2 -肾上腺素能受体介导的。由于去甲肾上腺素对β2 -肾上腺素能受体的亲和力较低，去甲肾上腺素诱导NK细胞脱离EC的效果不如EPI。福斯克林直接激活腺苷酸环化酶的结果与EPI相似，表明通过cAMP信号传导是诱导NK细胞脱离EC的必要条件。本研究的结果支持了一种假设，即儿茶酚胺通过β2‐肾上腺素能受体，通过改变NK细胞与EC之间的粘附相互作用，诱导NK细胞从边缘池向循环池募集。
Physical exercise, mental stress, or infusion of β‐adrenergic agonists result in an increase in the number of natural killer (NK) cells in the peripheral circulation. In view of the specific migration pattern of NK cells in vivo, it has been suggested that these cells may be released from the marginating pool in blood vessels. In the present report, the in vitro effect of catecholamines on the adhesion of NK cells to unstimulated human endothelial cells (EC) was characterized. Peripheral blood mononuclear cells were allowed to adhere to monolayers of EC, after which the adherent lymphocyte fraction was analyzed phenotypically by flow cytometry. NK cells were found to adhere preferentially to EC, a process that was reversed by the addition of various adrenergic agonists. Catecholamines selectively affected adhesion of NK cells and had no effect on T cell adhesion to EC, as was determined by the use of purified cell populations. Detachment of NK cells from EC could be achieved by short incubations (5 min) with epinephrine (EPI) and was concentration‐dependent, with an ED50 of 2 × 10 −10M. Using a panel of α and β‐adrenergic agonists and antagonists, we show that the detachment of NK cells is mediated via β2‐adrenergic receptors. In line with the lower affinity for β2‐adrenergic receptors, norepinephrine was less effective than EPI in inducing detachment of NK cells from EC. Direct activation of adenylate‐cyclase with forskolin gave similar results as observed with EPI, indicating that signaling through cAMP is necessary to induce detachment of NK cells from EC. The results of the present study lend support to the hypothesis that catecholamines, via β2‐adrenergic receptors, can induce recruitment of NK cells from the marginating pool to the circulating pool, by changing the adhesive interactions between NK cells and EC.
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体外结合人微血管内皮细胞的淋巴细胞的表型和功能特征。
DOI：
10.1172/jci113007
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Bender,JR;Pardi,R;Karasek,MA;Engleman,EG
通讯作者：
Engleman,EG
小鼠自然杀伤（NK）淋巴细胞表面层粘连蛋白样物质的表达及其在NK识别肿瘤靶细胞中的作用。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hiserodt,JC;Laybourn,KA;Varani,J
通讯作者：
Varani,J
人肺中的自然杀伤细胞功能是分开的。
DOI：
10.1164/arrd.1987.135.4.941
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Weissler,JC;Nicod,LP;Lipscomb,MF;Toews,GB
通讯作者：
Toews,GB
前列腺素 (PGE2) 和乙醇对自然杀伤细胞溶解过程的双重作用：效应器激活和 NK 细胞-靶细胞缀合物溶解抑制。
DOI：
--
发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kendall,RA;Targan,S
通讯作者：
Targan,S
影响因子：
3.3
作者：
B. Naliboff;D. Benton;G. Solomon;J. Morley;J. Fahey;E. Bloom;T. Makinodan;S. Gilmore
通讯作者：
B. Naliboff;D. Benton;G. Solomon;J. Morley;J. Fahey;E. Bloom;T. Makinodan;S. Gilmore