Characterization of the genuine type 2 chromatic acclimation in the two Geminocystis cyanobacteria.
Characterization of the genuine type 2 chromatic acclimation in the two Geminocystis cyanobacteria.
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DOI：
10.1093/dnares/dsx011
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发表时间：
2017-08-01
期刊：
影响因子：
--
通讯作者：
Eki T
中科院分区：
文献类型：
--
作者：
Hirose Y;Misawa N;Yonekawa C;Nagao N;Watanabe M;Ikeuchi M;Eki T
某些蓝细菌可以通过一个称为色适应（CA）的过程重塑其光合天线复合体藻胆体来调节它们吸收的光的波长。虽然已经报道了几种类型的CA，但在蓝藻门中CA的分子机制的多样性尚未完全了解。在这里，我们表征了双生囊藻菌株国家环境研究所（NIES）-3708和NIES-3709的CA的分子过程。吸收和荧光光谱显示，这两种菌株显着改变其藻红蛋白含量响应绿色和红色光。全基因组比较显示，这两个菌株共享的典型藻胆体结构组成的中央核心和周边杆，但它们不同的杆连接器的藻红蛋白的数量，从而具有不同的能力藻红蛋白的积累。RNA测序分析表明，藻胆体中藻红蛋白杆的长度受绿色光和红光敏感的CcaS/R系统的严格调控，而藻胆体的总数则受藻胆体和光系统之间的兴奋平衡系统的调控。我们根据基因组信息对传统的CA类型进行重新分类，并将这两种菌株的CA指定为真正的2型，其中藻红蛋白的组分而不是藻蓝蛋白的杆-膜连接子受CcaS/R系统的调节。
Certain cyanobacteria can adjust the wavelengths of light they absorb by remodeling their photosynthetic antenna complex phycobilisome via a process called chromatic acclimation (CA). Although several types of CA have been reported, the diversity of the molecular mechanisms of CA among the cyanobacteria phylum is not fully understood. Here, we characterized the molecular process of CA of Geminocystis sp. strains National Institute of Environmental Studies (NIES)-3708 and NIES-3709. Absorption and fluorescence spectroscopy revealed that both strains dramatically alter their phycoerythrin content in response to green and red light. Whole-genome comparison revealed that the two strains share the typical phycobilisome structure consisting of a central core and peripheral rods, but they differ in the number of rod linkers of phycoerythrin and thus have differing capacity for phycoerythrin accumulation. RNA sequencing analysis suggested that the length of phycoerythrin rods in each phycobilisome is strictly regulated by the green light and red light-sensing CcaS/R system, whereas the total number of phycobilisomes is governed by the excitation-balancing system between phycobilisomes and photosystems. We reclassify the conventional CA types based on the genome information and designate CA of the two strains as genuine type 2, where components of phycoerythrin, but not rod-membrane linker of phycocyanin, are regulated by the CcaS/R system.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.6
作者：
Li, L;Kehoe, DM
通讯作者：
Kehoe, DM
影响因子：
4.9
作者：
Narikawa, Rei;Suzuki, Fumiko;Ikeuchi, Masahiko
通讯作者：
Ikeuchi, Masahiko
影响因子：
11.6
作者：
Alvey, RM;Karty, JA;Kehoe, DM
通讯作者：
Kehoe, DM
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.