Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development.
Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development.
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甜橙 [Citrus sinensis (L.) Osbeck] 果实发育过程中自发突变体的转录组分析
DOI：
10.1093/jxb/ern329
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发表时间：
2009
影响因子：
6.9
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Liu Q;Zhu A;Chai L;Zhou W;Yu K;Ding J;Xu J;Deng X
柑橘中经常出现芽变。突变体的筛选是柑橘最重要的育种途径之一。然而，芽变的分子基础很少被研究。为了鉴定一个导致果实中番茄红素积累、柠檬酸含量低和蔗糖含量高的甜橙[C. sinensis (L.) Osbeck]自发芽变中差异表达的基因，采用抑制性消减杂交和微阵列分析来解读果实发育过程中的这种芽变。对差异表达的克隆进行测序后，共获得267个非冗余转录本，其中182个（68.2%）与已知基因产物具有同源性（E值≤1×10⁻¹⁰）。在果实发育过程中，芽变中很少有基因组成性地上调或下调（倍数变化≥2）。自组织树算法分析结果表明，95.1%的差异表达基因与番茄红素积累的起始广泛协调。代谢过程、细胞过程、定位的建立、对刺激的反应以及生物调节相关的转录本是受调控最多的基因之一。这些基因参与了许多生物过程，如有机酸代谢、脂质代谢、运输和丙酮酸代谢等。此外，开花后170天差异调控的13个基因与先前描述的信号转导或转录因子具有同源性。本研究中产生的信息为理解柑橘芽变提供了新的线索。
Bud mutations often arise in citrus. The selection of mutants is one of the most important breeding channels in citrus. However, the molecular basis of bud mutation has rarely been studied. To identify differentially expressed genes in a spontaneous sweet orange [C. sinensis (L.) Osbeck] bud mutation which causes lycopene accumulation, low citric acid, and high sucrose in fruit, suppression subtractive hybridization and microarray analysis were performed to decipher this bud mutation during fruit development. After sequencing of the differentially expressed clones, a total of 267 non-redundant transcripts were obtained and 182 (68.2%) of them shared homology (E-value ≤1×10−10) with known gene products. Few genes were constitutively up- or down-regulated (fold change ≥2) in the bud mutation during fruit development. Self-organizing tree algorithm analysis results showed that 95.1% of the differentially expressed genes were extensively coordinated with the initiation of lycopene accumulation. Metabolic process, cellular process, establishment of localization, response to stimulus, and biological regulation-related transcripts were among the most regulated genes. These genes were involved in many biological processes such as organic acid metabolism, lipid metabolism, transport, and pyruvate metabolism, etc. Moreover, 13 genes which were differentially regulated at 170 d after flowering shared homology with previously described signal transduction or transcription factors. The information generated in this study provides new clues to aid in the understanding of bud mutation in citrus.
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影响因子：
5.4
作者：
Bernet, GP;Asíns, MJ
通讯作者：
Asíns, MJ
影响因子：
9.4
作者：
Derory, J;Léger, P;Kremer, A
通讯作者：
Kremer, A
DOI：
10.1017/s0016672303006177
发表时间：
2003-06-01
期刊：
GENETICAL RESEARCH
影响因子：
--
作者：
Aradhya, MK;Dangl, GS;Simon, CJ
通讯作者：
Simon, CJ
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.1
作者：
Bretó, MP;Ruiz, C;Asíns, MJ
通讯作者：
Asíns, MJ