Zebrafish nephrosin helps host defence against Escherichia coli infection.
Zebrafish nephrosin helps host defence against Escherichia coli infection.
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斑马鱼肾素有助于宿主防御大肠杆菌感染
DOI：
10.1098/rsob.170040
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发表时间：
2017-08
期刊：
影响因子：
5.8
通讯作者：
Zhang Y
中科院分区：
文献类型：
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作者：
Di Q;Lin Q;Huang Z;Chi Y;Chen X;Zhang W;Zhang Y
中性粒细胞在天然免疫中起重要作用，主要依靠各种含酶颗粒杀灭吞噬的微生物。斑马鱼肾素（npsn）特异性表达于中性粒细胞，然而，其功能在很大程度上是未知的。在这里，我们通过CRISPR/Cas9产生了npsn突变体（npsnsmu5）以研究Npsn的体内功能。中性粒细胞的总体发育和数量在npsn缺陷突变体中保持不变，而中性粒细胞的抗菌功能是有缺陷的。感染大肠杆菌后，与野生型胚胎相比，npsnsmu5突变体表现出较低的存活率和更严重的细菌负荷，以及对感染挑战的炎症反应增强，而npsn过表达的斑马鱼表现出增强的宿主防御大肠杆菌。大肠杆菌感染。这些发现表明，斑马鱼Npsn促进宿主防御细菌感染。此外，我们的研究结果表明，npsn缺陷和过度表达斑马鱼可能作为体内先天免疫的有效模型。
Neutrophils play important roles in innate immunity and are mainly dependent on various enzyme-containing granules to kill engulfed microorganisms. Zebrafish nephrosin (npsn) is specifically expressed in neutrophils; however, its function is largely unknown. Here, we generated an npsn mutant (npsnsmu5) via CRISPR/Cas9 to investigate the in vivo function of Npsn. The overall development and number of neutrophils remained unchanged in npsn-deficient mutants, whereas neutrophil antibacterial function was defective. Upon infection with Escherichia coli, the npsnsmu5 mutants exhibited a lower survival rate and more severe bacterial burden, as well as augmented inflammatory response to challenge with infection when compared with wild-type embryos, whereas npsn-overexpressing zebrafish exhibited enhanced host defence against E. coli infection. These findings demonstrated that zebrafish Npsn promotes host defence against bacterial infection. Furthermore, our findings suggested that npsn-deficient and -overexpressing zebrafish might serve as effective models of in vivo innate immunity.
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影响因子：
4.3
作者：
Nguyen-Chi M;Phan QT;Gonzalez C;Dubremetz JF;Levraud JP;Lutfalla G
通讯作者：
Lutfalla G
影响因子：
4
作者：
Colucci-Guyon, Emma;Tinevez, Jean-Yves;Herbomel, Philippe
通讯作者：
Herbomel, Philippe
影响因子：
4.8
作者：
Hung, CH;Huang, HR;Chang, GD
通讯作者：
Chang, GD
DOI：
10.1007/978-1-4614-0106-3_15
发表时间：
2012-01-01
期刊：
CURRENT TOPICS IN INNATE IMMUNITY II
影响因子：
--
作者：
Novoa, Beatriz;Figueras, Antonio
通讯作者：
Figueras, Antonio
影响因子：
44.1
作者：
通讯作者：
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