LAR receptor tyrosine phosphatases and HSPGs guide peripheral sensory axons to the skin.
LAR receptor tyrosine phosphatases and HSPGs guide peripheral sensory axons to the skin.
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DOI：
10.1016/j.cub.2012.01.040
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发表时间：
2012-03-06
期刊：
影响因子：
9.2
通讯作者：
Sagasti, Alvaro
中科院分区：
文献类型：
--
作者：
Wang, Fang;Wolfson, Sean N.;Gharib, Arash;Sagasti, Alvaro
Peripheral axons of somatosensory neurons innervate the skin early in development to detect touch stimuli. Embryological experiments had suggested that the skin produces guidance cues that attract sensory axons, but neither the attractants nor their neuronal receptors had previously been identified. To investigate peripheral axon navigation to the skin, we combined live imaging of developing zebrafish Rohon-Beard (RB) neurons with molecular loss-of-function manipulations. Simultaneously knocking down two members of the LAR family of receptor tyrosine phosphatases expressed in RB neurons, or inhibiting their function with dominant negative proteins, misrouted peripheral axons to internal tissues. Time-lapse imaging indicated that peripheral axon guidance, rather than outgrowth or maintenance, was defective in LAR deficient neurons. Peripheral axons displayed a similar misrouting phenotype in mutants defective in heparan sulfate proteoglycan (HSPG) production and avoided regions in which HSPGs were locally degraded. HSPGs and LAR family receptors are required for sensory axon guidance to the skin. Together, our results support a model in which peripheral HSPGs are attractive ligands for LAR receptors on RB neurons.
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影响因子：
2.5
作者：
O'Brien, Georgeann S.;Rieger, Sandra;Wang, Fang;Smolen, Gromoslaw A.;Gonzalez, Robert E.;Buchanan, JoAnn;Sagasti, Alvaro
通讯作者：
Sagasti, Alvaro
DOI：
10.3791/1129
发表时间：
2009-02-16
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
O'Brien, Georgeann S;Rieger, Sandra;Sagasti, Alvaro
通讯作者：
Sagasti, Alvaro
影响因子：
5.3
作者：
Liu, Y;Halloran, MC
通讯作者：
Halloran, MC
影响因子：
64.5
作者：
Krueger, NX;VanVactor, D;Saito, H
通讯作者：
Saito, H
影响因子：
16.2
作者：
Clandinin, TR;Lee, CH;Zipursky, SL
通讯作者：
Zipursky, SL