Melatonin Modulates Lipid Metabolism in Porcine Cumulus-Oocyte Complex via Its Receptors.
Melatonin Modulates Lipid Metabolism in Porcine Cumulus-Oocyte Complex via Its Receptors.
复制标题
褪黑素通过受体调节猪卵丘细胞复合体的脂质代谢。
DOI：
10.3389/fcell.2021.648209
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发表时间：
2021
影响因子：
5.5
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Zhu T;Guan S;Lv D;Zhao M;Yan L;Shi L;Ji P;Zhang L;Liu G
脂质是哺乳动物卵母细胞的重要能量来源。褪黑素对猪卵母细胞体外成熟有促进作用，但其作用机制尚不清楚。本研究首次建立了利用Lipi-Blue和Lipi-Green探针检测单个活卵母细胞脂质代谢的方法。结果表明，成熟卵母细胞中既有脂类合成，也有脂类分解，但总体水平下降。然后用褪黑激素或脂质代谢相关的抑制剂（三酸甘油酯C，鱼藤酮，或etomoxir）处理用探针染色的成熟卵母细胞。结果表明，猪卵母细胞的脂质代谢和成熟均受到破坏，褪黑激素对三酸甘油酯C或鱼藤酮处理的卵母细胞有拯救作用，而对依托莫西处理的卵母细胞无拯救作用。进一步的研究表明，卵丘细胞能够通过间隙连接将脂质转移到卵母细胞。此外，还观察到卵丘细胞中存在褪黑激素受体，并且是卵母细胞维持脂质代谢所必需的。同时，褪黑素还对卵丘细胞的整体基因表达有调节作用，尤其是抗氧化剂（SOD 1、GPX 1、GPX 3、GPX 4、PRDX 2和PRDX 5）、脂质代谢（FABP 3、FABP 5、ACACB、TECR等）、线粒体呼吸（GPD 1、ETFB、CYC 1和ATP合成酶基因）。目前的研究表明，褪黑素通过其卵丘细胞受体调节成熟卵母细胞的脂质代谢，有利于卵母细胞的发育能力。
Lipid is a crucial energy resource for mammalian oocyte. Melatonin could benefit the maturation of porcine oocyte in vitro, but the related mechanism is not elucidated yet. In the current study, methods to monitor lipid metabolism in single live oocytes were firstly established using probes (Lipi-Blue and Lipi-Green). It was observed that both lipid biogenesis and lipolysis occurred in maturing oocyte, but the general level of lipids dropped. Then maturing oocytes stained with probes were treated with melatonin or lipid metabolic-related inhibitors (triacsin C, rotenone, or etomoxir). The results showed that the lipid metabolism and maturation of porcine oocytes were all disrupted and that melatonin rescued the oocytes treated with triacsin C or rotenone, but not those treated with etomoxir. Further investigation demonstrated that cumulus cells are able to transfer lipids to oocytes via gap junctions. It was also observed that melatonin receptors exist in cumulus cells and are required for oocytes to maintain lipid metabolism. Meanwhile, the global gene expressing in cumulus cells was also modulated by melatonin, especially the genes related to antioxidants (SOD1, GPX1, GPX3, GPX4, PRDX2, and PRDX5), lipid metabolism (FABP3, FABP5, ACACB, TECR, etc.), and mitochondrial respiration (GPD1, ETFB, CYC1, and the genes of ATP synthase). Altogether the current research demonstrates that melatonin modulates lipid metabolism in maturing oocytes through its receptors in cumulus cells and benefits the developmental competence of oocytes.
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