Stimulatory Effects of Melatonin on Porcine In Vitro Maturation Are Mediated by MT2 Receptor.
Stimulatory Effects of Melatonin on Porcine In Vitro Maturation Are Mediated by MT2 Receptor.
复制标题
DOI：
10.3390/ijms19061581
复制
发表时间：
2018-05-26
影响因子：
5.6
通讯作者：
Lee BC
中科院分区：
文献类型：
--
作者：
Lee S;Jin JX;Taweechaipaisankul A;Kim GA;Lee BC
褪黑激素是一种具有多种生物活性的多功能分子。褪黑素通过MT 2受体调节猪颗粒细胞的功能，提示MT 2受体介导褪黑素促进猪卵丘-卵母细胞复合体（COCs）卵丘扩张的可能性。因此，我们研究了MT 2在猪COCs中的存在，以及褪黑素与或不与选择性MT 2拮抗剂（luzindole和4-P-PDOT）对这一过程的影响; COCs经历了六种不同条件（对照、褪黑素、luzindole、4-P-PDOT、褪黑素+ luzindole或褪黑素+ 4-P-PDOT）的体外成熟培养。卵丘扩张，卵母细胞核成熟，和随后的胚胎发育后孤雌激活（PA）进行了评价。在实验1中，MT 2在卵母细胞和卵丘细胞中表达。在实验2中，褪黑激素显着增加的比例完全卵丘扩展（度4），这是抑制同时添加luzindole或4-P-PDOT。在与卵丘扩张、细胞凋亡和MT 2相关的基因表达中观察到类似的模式。在实验3中，未成熟卵母细胞率、退化卵母细胞率和MII卵母细胞率在各组之间没有显著差异。在实验4中，褪黑激素显着增加囊胚形成率和总囊胚细胞数后PA，但这些影响被取消时，无论是luzindole或4-P-PDOT伴随加入。总之，我们的研究结果表明，MT 2受体介导的褪黑素对猪卵丘扩张和随后的胚胎发育的刺激作用。
Melatonin is a multifunctional molecule with numerous biological activities. The fact that melatonin modulates the functions of porcine granulosa cells via the MT2 receptor suggests the possibility of MT2 receptor-mediation for melatonin to promote cumulus expansion of porcine cumulus-oocyte complexes (COCs). Therefore, we investigated the presence of MT2 in porcine COCs, and the effects of melatonin with or without selective MT2 antagonists (luzindole and 4-P-PDOT) on this process; COCs underwent in vitro maturation culturing with six different conditions (control, melatonin, luzindole, 4-P-PDOT, melatonin + luzindole or melatonin + 4-P-PDOT). Cumulus expansion, oocyte nuclear maturation, and subsequent embryo development after parthenogenetic activation (PA) were evaluated. In experiment 1, MT2 was expressed in both oocytes and cumulus cells. In experiment 2, melatonin significantly increased the proportion of complete cumulus expansion (degree 4), which was inhibited by simultaneous addition of either luzindole or 4-P-PDOT. A similar pattern was observed in the expression of genes related to cumulus expansion, apoptosis, and MT2. In experiment 3, no significant difference was observed in immature, degenerate, and MII oocyte rates among the groups. In experiment 4, melatonin significantly increased blastocyst formation rates and total blastocyst cell numbers after PA, but these effects were abolished when either luzindole or 4-P-PDOT was added concomitantly. In conclusion, our results indicate that the MT2 receptor mediated the stimulatory effects of melatonin on porcine cumulus expansion and subsequent embryo development.
登录
查看更多内容
影响因子：
2.5
作者：
CHEN, L;RUSSELL, PT;LARSEN, WJ
通讯作者：
LARSEN, WJ
培养的大鼠皮质星形胶质细胞合成褪黑激素：缺乏昼夜节律
影响因子：
10.3
作者：
Liu, Ya-Jing;Zhuang, Jun;Zhou, Jiang-Ning
通讯作者：
Zhou, Jiang-Ning
影响因子：
6.7
作者：
Reiter, Russel J.;Tamura, Hiroshi;Xu, Xiao-Ying
通讯作者：
Xu, Xiao-Ying
影响因子：
2.5
作者：
El-Raey, Mohamed;Geshi, Masaya;Nagai, Takashi
通讯作者：
Nagai, Takashi
影响因子：
2.8
作者：
Lee, Sanghoon;Park, Eun Jung;Lee, Byeong Chun
通讯作者：
Lee, Byeong Chun