West Nile Virus Vaccination Protects against Usutu Virus Disease in Mice.
West Nile Virus Vaccination Protects against Usutu Virus Disease in Mice.
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西尼罗河病毒疫苗对小鼠的Usutu病毒病具有保护作用。
DOI：
10.3390/v13122352
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发表时间：
2021-11-23
期刊：
影响因子：
--
通讯作者：
Duggal NK
中科院分区：
文献类型：
--
作者：
Salgado R;Hawks SA;Frere F;Vázquez A;Huang CY;Duggal NK
西尼罗河病毒（WNV）和西尼罗河病毒（USUV）是蚊媒黄病毒，可导致人类神经侵入性疾病。西尼罗河病毒和USUV在非洲和欧洲传播，关系密切。由于抗原相似性，WNV特异性抗体和UUV特异性抗体具有结合异源病毒的潜力;然而，尚不清楚这种相互作用是否可以提供针对感染的保护。为了研究先前的WNV暴露如何影响USUV感染，我们使用了一种减毒WNV疫苗，该疫苗在登革病毒2型疫苗株的骨架中含有WNV的表面蛋白，并可预防WNV疾病。我们假设接种这种减毒WNV疫苗可以预防USUV感染。接种疫苗后，使用血清在体外测量针对WNV和USUV的中和应答。来自接种CD-1和Ifnar 1 −/−小鼠的血清与WNV和USUV交叉中和。然后用非洲或欧洲USUV毒株对所有小鼠进行攻击。在CD-1小鼠中，接种和模拟接种小鼠之间的USUV滴度没有差异。然而，在Ifnar 1 −/−模型中，与模拟疫苗接种小鼠相比，接种疫苗的小鼠具有显着更高的存活率和显着更低的USUV病毒血症。我们的研究结果表明，暴露于一种减毒形式的西尼罗河病毒保护对严重的USUV疾病的小鼠和elevens中和反应，以西尼罗河病毒和USUV。未来的研究将调查负责保护免受USUV感染的免疫机制诱导的WNV疫苗接种，提供关键的见解，将是必不可少的USUV和WNV疫苗的发展。
West Nile virus (WNV) and Usutu virus (USUV) are mosquito-borne flaviviruses that can cause neuroinvasive disease in humans. WNV and USUV circulate in both Africa and Europe and are closely related. Due to antigenic similarity, WNV-specific antibodies and USUV-specific antibodies have the potential to bind heterologous viruses; however, it is unclear whether this interaction may offer protection against infection. To investigate how prior WNV exposure would influence USUV infection, we used an attenuated WNV vaccine that contains the surface proteins of WNV in the backbone of a dengue virus 2 vaccine strain and protects against WNV disease. We hypothesized that vaccination with this attenuated WNV vaccine would protect against USUV infection. Neutralizing responses against WNV and USUV were measured in vitro using sera following vaccination. Sera from vaccinated CD-1 and Ifnar1−/− mice cross-neutralized with WNV and USUV. All mice were then subsequently challenged with an African or European USUV strain. In CD-1 mice, there was no difference in USUV titers between vaccinated and mock-vaccinated mice. However, in the Ifnar1−/− model, vaccinated mice had significantly higher survival rates and significantly lower USUV viremia compared to mock-vaccinated mice. Our results indicate that exposure to an attenuated form of WNV protects against severe USUV disease in mice and elicits a neutralizing response to both WNV and USUV. Future studies will investigate the immune mechanisms responsible for the protection against USUV infection induced by WNV vaccination, providing critical insight that will be essential for USUV and WNV vaccine development.
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影响因子：
3.8
作者：
Kuchinsky SC;Hawks SA;Mossel EC;Coutermarsh-Ott S;Duggal NK
通讯作者：
Duggal NK
影响因子：
3.7
作者：
Calzolari M;Bonilauri P;Bellini R;Albieri A;Defilippo F;Maioli G;Galletti G;Gelati A;Barbieri I;Tamba M;Lelli D;Carra E;Cordioli P;Angelini P;Dottori M
通讯作者：
Dottori M
欧洲和非洲的新兴arbovirus忽视了USUTU病毒的进化史和扩散的重建。
影响因子：
6.4
作者：
Engel D;Jöst H;Wink M;Börstler J;Bosch S;Garigliany MM;Jöst A;Czajka C;Lühken R;Ziegler U;Groschup MH;Pfeffer M;Becker N;Cadar D;Schmidt-Chanasit J
通讯作者：
Schmidt-Chanasit J
影响因子：
3.7
作者：
Blazquez, Ana-Belen;Escribano-Romero, Estela;Saiz, Juan-Carlos
通讯作者：
Saiz, Juan-Carlos
影响因子：
5.4
作者：
Butrapet, S;Huang, CYH;Kinney, RM
通讯作者：
Kinney, RM