Small Circular DNA Molecules as Triangular Scaffolds for the Growth of 3D Single Crystals
Small Circular DNA Molecules as Triangular Scaffolds for the Growth of 3D Single Crystals
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小圆形 DNA 分子作为 3D 单晶生长的三角形支架
DOI：
10.3390/biom10060814
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发表时间：
2020-05
期刊：
影响因子：
5.5
通讯作者：
Xiao Shou-Jun
中科院分区：
文献类型：
--
作者：
Wang Yu;Guo Xin;Kou Bo;Zhang Ling;Xiao Shou-Jun
DNA是一种非常有用的分子，用于程序化自组装三维（三维）纳米结构。有组织的3D DNA组装和晶体使科学家能够进行许多应用的研究，如酶催化，生物免疫分析和光活性。Seeman和他的同事报道了第一个自组装的3D DNA单晶，基于一个刚性三角形瓦片，瓦片边长为两圈。直到今天，通过编程自组装成功设计的3D单晶是可数的，并且仍然是DNA纳米技术中最具挑战性的任务，这是由于刚性瓦片和精确包装的高度约束条件。我们在这里报道了使用小的环状DNA分子代替线性分子作为核心三角形支架来生长3D单晶。几个结晶参数进行了筛选，DNA浓度，孵育时间，水蒸气交换速度，和pH值的采样缓冲液。在宏观尺度上获得了几种不同形貌的DNA单晶。晶体可以提供内部孔隙，用于容纳Cy3和Cy5标记的三链体形成寡核苷酸（TFO）的客体分子。小的环状DNA分子在自组装3D单晶中的成功鼓励它们在DNA纳米技术中的使用，这与环状瓦片的刚性、稳定性和柔性有关。
DNA is a very useful molecule for the programmed self-assembly of 3D (three dimension) nanoscale structures. The organised 3D DNA assemblies and crystals enable scientists to conduct studies for many applications such as enzymatic catalysis, biological immune analysis and photoactivity. The first self-assembled 3D DNA single crystal was reported by Seeman and his colleagues, based on a rigid triangle tile with the tile side length of two turns. Till today, successful designs of 3D single crystals by means of programmed self-assembly are countable, and still remain as the most challenging task in DNA nanotechnology, due to the highly constrained conditions for rigid tiles and precise packing. We reported here the use of small circular DNA molecules instead of linear ones as the core triangle scaffold to grow 3D single crystals. Several crystallisation parameters were screened, DNA concentration, incubation time, water-vapour exchange speed, and pH of the sampling buffer. Several kinds of DNA single crystals with different morphologies were achieved in macroscale. The crystals can provide internal porosities for hosting guest molecules of Cy3 and Cy5 labelled triplex-forming oligonucleotides (TFOs). Success of small circular DNA molecules in self-assembling 3D single crystals encourages their use in DNA nanotechnology regarding the advantage of rigidity, stability, and flexibility of circular tiles.
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