A conserved female-specific larval requirement for MtnB function facilitates sex separation in multiple species of disease vector mosquitoes.
A conserved female-specific larval requirement for MtnB function facilitates sex separation in multiple species of disease vector mosquitoes.
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DOI：
10.1186/s13071-021-04844-w
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发表时间：
2021-06-26
影响因子：
3.2
通讯作者：
Duman-Scheel M
中科院分区：
文献类型：
--
作者：
Mysore K;Sun L;Roethele JB;Li P;Igiede J;Misenti JK;Duman-Scheel M
预计性别特异性基因座簇会形成登革热媒介蚊子埃及伊蚊的 M/m 性别决定基因座的边界，但这些基因的身份尚不清楚。这些位点的鉴定和表征可以促进对蚊子性染色体进化的更好理解，并有助于阐明雄性蚊子性别分离的新策略，这是依赖于雄性蚊子的大规模饲养和释放的几种新兴蚊子种群控制策略的要求。这项研究表明，甲基硫核酮糖-1-磷酸脱水酶 (MtnB) 基因位于 M/m 基因座附近，编码蛋氨酸补救途径的进化保守成分，是雌性幼虫生存所必需的。幼虫食用经过改造可表达与 MtnB 相对应的干扰 RNA 的酿酒酵母（酵母）菌株会导致靶基因沉默和雌性显着死亡，但对雄性埃及伊蚊的生存或健康没有影响。将酵母杀幼虫剂整合到大规模培养方案中，可以规模化生产健康的成年雄性蚊子。此外，沉默白纹伊蚊、冈比亚按蚊和致倦库蚊中的 MtnB 直向同源物揭示了不同种类蚊子中雌性特异性幼虫对 MtnB 的保守需求。这项研究的结果可能对蚊子性染色体进化的研究具有重要意义，表明沉默 MtnB 可以促进多种病媒昆虫的性别分离。在线版本包含可在 10.1186/s13071-021-04844-w 获取的补充材料。
Clusters of sex-specific loci are predicted to shape the boundaries of the M/m sex-determination locus of the dengue vector mosquito Aedes aegypti, but the identities of these genes are not known. Identification and characterization of these loci could promote a better understanding of mosquito sex chromosome evolution and lead to the elucidation of new strategies for male mosquito sex separation, a requirement for several emerging mosquito population control strategies that are dependent on the mass rearing and release of male mosquitoes. This investigation revealed that the methylthioribulose-1-phosphate dehydratase (MtnB) gene, which resides adjacent to the M/m locus and encodes an evolutionarily conserved component of the methionine salvage pathway, is required for survival of female larvae. Larval consumption of Saccharomyces cerevisiae (yeast) strains engineered to express interfering RNA corresponding to MtnB resulted in target gene silencing and significant female death, yet had no impact on A. aegypti male survival or fitness. Integration of the yeast larvicides into mass culturing protocols permitted scaled production of fit adult male mosquitoes. Moreover, silencing MtnB orthologs in Aedes albopictus, Anopheles gambiae, and Culex quinquefasciatus revealed a conserved female-specific larval requirement for MtnB among different species of mosquitoes. The results of this investigation, which may have important implications for the study of mosquito sex chromosome evolution, indicate that silencing MtnB can facilitate sex separation in multiple species of disease vector insects. The online version contains supplementary material available at 10.1186/s13071-021-04844-w.
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影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z
影响因子：
3.8
作者：
Juneja P;Osei-Poku J;Ho YS;Ariani CV;Palmer WJ;Pain A;Jiggins FM
通讯作者：
Jiggins FM
影响因子：
46.9
作者：
Kyrou K;Hammond AM;Galizi R;Kranjc N;Burt A;Beaghton AK;Nolan T;Crisanti A
通讯作者：
Crisanti A
影响因子：
2.7
作者：
Hill, Casey L.;Sharma, Avinash;Shouche, Yogesh;Severson, David W.
通讯作者：
Severson, David W.