C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog.
C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog.
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DOI：
10.1038/nn.2540
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发表时间：
2010-06
影响因子：
25
通讯作者：
Xu, X. Z. Shawn
中科院分区：
文献类型：
--
作者：
Liu, Jie;Ward, Alex;Gao, Jingwei;Dong, Yongming;Nishio, Nana;Inada, Hitoshi;Kang, Lijun;Yu, Yong;Ma, Di;Xu, Tao;Mori, Ikue;Xie, Zhixiong;Xu, X. Z. Shawn
无眼动物线虫具有光感，由感光细胞介导的趋光行为。然而，线虫光转导的分子和细胞机制在很大程度上仍不清楚。通过记录野生型和各种突变型蠕虫的光感受器神经元ASJ，我们证明ASJ中的光转导是一个G蛋白介导的过程，需要膜相关的鸟氨酸环化酶而不是典型的cGMP裂解磷酸二酯酶(PDE)。此外，我们发现线虫的光转导需要LITE-1，这是一种已知的无脊椎动物味觉受体家族成员的候选光受体蛋白。遗传学、药理学和电生理数据表明，LITE-1通过G蛋白信号转导ASJ中的光信号，导致第二信使cGMP上调，随后打开cGMP敏感的CNG通道，从而刺激光感受器细胞。我们的结果在线虫体内发现了一个光转导级联反应，并暗示了“味觉受体”在光转导中的作用。
The “eyeless” animal C. elegans possesses the sense of light and engages in phototaxis behavior mediated by photoreceptor cells. However, the molecular and cellular mechanisms underlying phototransduction in C. elegans remain largely unclear. By recording the photoreceptor neuron ASJ in wild-type and various mutant worms, here we show that phototransduction in ASJ is a G protein-mediated process and requires membrane-associated guanylate cyclases but not typical cGMP-cleaving phosphodiesterases (PDEs). In addition, we find that C. elegans phototransduction requires LITE-1, a candidate photoreceptor protein known to be a member of the invertebrate taste receptor family. Genetic, pharmacological and electrophysiological data suggest a model whereby LITE-1 transduces light signals in ASJ through G-protein signaling, which leads to up-regulation of the second messenger cGMP followed by opening of cGMP-sensitive CNG channels and thereby stimulation of photoreceptor cells. Our results identify a phototransduction cascade in C. elegans and implicate the function of a “taste receptor" in phototransduction.
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