Food-entrained rhythmic expression of apolipoprotein E expression in the hypothalamus of rats.
Food-entrained rhythmic expression of apolipoprotein E expression in the hypothalamus of rats.
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DOI：
10.1016/j.brainres.2009.04.004
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发表时间：
2009-06-01
期刊：
影响因子：
2.9
通讯作者：
Liu M
中科院分区：
文献类型：
--
作者：
Shen L;Carey K;Wang DQ;Woods SC;Liu M
载脂蛋白E(ApoE)是一种主要在肝脏和大脑合成的蛋白质。为了进一步了解脑载脂蛋白E在日常食物摄入管理中的作用，我们研究了自由喂养(FF)和食物限制(RF)大鼠下丘脑载脂蛋白E基因和蛋白表达的昼夜规律。在FF大鼠，下丘脑apoE基因和蛋白水平有波动，峰值出现在暗期，最低点出现在亮期。这一模式在RF大鼠中发生了改变，在光照阶段的4小时喂养期间，RF大鼠下丘脑apoE mRNA和蛋白水平显著增加。虽然皮质酮(CORT)水平在时间上与下丘脑apoE的增高期一致，但肾上腺切除(ADX)耗竭CORT对下丘脑apoE水平在这两个时期均无显著影响，提示下丘脑apoE的昼夜节律模式受循环CORT以外的其他因素的调节。在正常的昼夜节律周期和限制摄食期，下丘脑apoE与摄食量呈正相关，提示下丘脑apoE是食物携带的，可能参与日常摄食量的生理调节。
Apolipoprotein E (apoE) is a protein mainly synthesized in the liver and brain. To further understand the role of brain apoE in the management of daily food intake, we have examined the circadian pattern of hypothalamic apoE gene and protein expression in freely-fed (FF) and food-restricted (RF, food provided 4 h daily between 1000 h and 1400 h) rats sacrificed at 3-h intervals throughout the light-dark cycle. In FF rats, hypothalamic apoE mRNA and protein levels fluctuated with peaks occuring during the dark phase and the nadirs occuring during the light phase. This pattern was altered in RF rats, which had a marked increase in hypothalamic apoE mRNA and protein levels during the 4-h feeding period in the light phase. Although corticosterone (CORT) levels temporally coincided with the increasing phase of apoE in the hypothalamus in both FF and RF rats, depletion of CORT by adrenalectomy (ADX) did not significantly influence the hypothalamic apoE levels during either period, implying that the circadian pattern of hypothalamic apoE is regulated by factors other than circulating CORT. The finding that hypothalamic apoE and food intake are positively associated during the normal circadian cycle as well as in the period of restricted feeding suggests that hypothalamic apoE is food-entrained and likely involved in the physiological regulation of daily food intake.
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