Mutation of amino acids within the gibbon ape leukemia virus (GALV) receptor differentially affects feline leukemia virus subgroup B, simian sarcoma-associated virus, and GALV infections
Mutation of amino acids within the gibbon ape leukemia virus (GALV) receptor differentially affects feline leukemia virus subgroup B, simian sarcoma-associated virus, and GALV infections
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长臂猿白血病病毒 (GALV) 受体内的氨基酸突变对猫白血病病毒 B 亚型、猿肉瘤相关病毒和 GALV 感染有不同影响
作者：
C. Tailor;Yasuhiro Takeuchi;B. O'hara;S. Johann;Robin A. Weiss;Mary Collins
三种C型逆转录病毒，即巨猿白血病病毒（GALV）、猿猴肉瘤相关病毒（SSAV）和猫白血病病毒亚组B（FeLV-B），通过与相同的细胞表面受体GLVR 1相互作用感染人类细胞。使用LacZ逆转录病毒假型和突变GLVR 1表达载体转染的鼠细胞，我们表明，相同的9个氨基酸的区域的人GLVR 1是至关重要的感染三种病毒。由于受体水平的阻断，大鼠细胞对LacZ（FeLV-B）假型感染不敏感。我们在大鼠GLVR 1的9个氨基酸关键区域中发现了与人GLVR 1的多个氨基酸差异。人-大鼠嵌合GLVR 1在鼠细胞中的表达表明，大鼠GLVR 1可以作为GALV和SSAV的受体发挥作用，但不作为FeLV-B的受体。大鼠GLVR 1关键区域中的人GLVR 1氨基酸置换确定了三种负责FeLV-B感染抗性的氨基酸;其中两种影响SSAV感染，但不影响GALV感染。
The three type C retroviruses, gibbon ape leukemia virus (GALV), simian sarcoma-associated virus (SSAV), and feline leukemia virus subgroup B (FeLV-B), infect human cells by interacting with the same cell surface receptor, GLVR1. Using LacZ retroviral pseudotypes and murine cells transfected with mutant GLVR1 expression vectors, we show that the same 9-amino-acid region of human GLVR1 is critical for infection by the three viruses. Rat cells were not susceptible to infection by LacZ (FeLV-B) pseudotypes because of a block at the receptor level. We found multiple amino acid differences from human GLVR1 in the 9-amino-acid critical region of rat GLVR1. Expression of a human-rat chimeric GLVR1 in murine cells demonstrated that rat GLVR1 could function as a receptor for GALV and SSAV but not for FeLV-B. Substitution of human GLVR1 amino acids in the critical region of rat GLVR1 identified three amino acids as responsible for resistance to FeLV-B infection; two of these affect SSAV infection, but none affects GALV infection.
DOI：
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发表时间：
1990-03
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子：
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作者：
O'hara;S. Johann;H. Klinger;D. Blair;H. Rubinson;K. Dunn;P. Sass;S. Vitek;T. Robins
通讯作者：
O'hara;S. Johann;H. Klinger;D. Blair;H. Rubinson;K. Dunn;P. Sass;S. Vitek;T. Robins
猫白血病病毒 (FeLV) A 亚组和内源 FeLV 元件之间的生物筛选重组体。
影响因子：
3.7
作者：
Sheets,RL;Pandey,R;Klement,V;Grant,CK;Roy-Burman,P
通讯作者：
Roy-Burman,P