Biologically selected recombinants between feline leukemia virus (FeLV) subgroup A and an endogenous FeLV element.
Biologically selected recombinants between feline leukemia virus (FeLV) subgroup A and an endogenous FeLV element.
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猫白血病病毒 (FeLV) A 亚组和内源 FeLV 元件之间的生物筛选重组体。
DOI：
10.1016/0042-6822(92)90924-e
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
Roy-Burman,P
中科院分区：
文献类型：
--
作者：
Sheets,RL;Pandey,R;Klement,V;Grant,CK;Roy-Burman,P
为了阐明猫白血病病毒（FeLV）感染过程中可能产生的近端白血病原，在体外细胞培养中检测了已知序列的分子克隆外源性和内源性FeLV原病毒之间的同源重组。在转染的猫成纤维细胞中，含有最常见FeLV亚群（FeLV亚群A或FeLV-A）感染性成员的质粒与含有内源性遗传FeLV样猫基因组元件包膜（env）基因的非感染性构建体共表达。选择产生的病毒是因为它们能够在人细胞中繁殖，并且能够抵抗亲本的亲生态FeLV-A或非传染性内源性构建体的感染。对由此衍生的重组体的分析发现，在所使用的选择条件下，envgene中有限数量的位点被优先用于重组felv的产生。这些位点聚集在enengene的表面糖蛋白（SU）部分，并且FeLV-A的大部分（但不是全部）SU基因产物，从n端开始，可以被来自内源性元素的序列所取代，仍然允许病毒具有生物活性。事实上，这些基因的替换使亲本FeLV-A的感染性从嗜生态性扩展到嗜多性细胞性。此外，在SU区域的替换产生了许多重组，其中FeLV-A的初级中和五肽表位由于其内源元件的变异而改变。在一些重组中，这个序列也被发现经常发生突变。与该抗体结合结构域的变化一致，重组病毒仅被针对该表位的单克隆抗体弱抑制，而FeLV-A对中和高度敏感。
In efforts to elucidate the proximal leukemogens that might be produced during a feline leukemia virus (FeLV) infection of cats, homologous recombinations between molecularly cloned exogenous and endogenous FeLV proviruses of known sequences were examined in cell culturesin vitro. A plasmid containing an infectious member of the most commonly occurring FeLV subgroup (FeLV subgroup A or FeLV-A) was coexpressed with noninfectious constructs containing the envelope (env) gene of an endogenously inherited FeLV-like feline genomic element in transfected feline fibroblasts. The viruses generated were selected for their ability to propagate in human cells which are resistant to infection by the parental ecotropic FeLV-A or the noninfectious endogenous constructs. An analysis of the recombinants thus derived identified a limited number of sites in theenvgene which were preferentially utilized in the generation of recombinant FeLVs under the selection conditions used. These sites were clustered in the surface glycoprotein (SU) moiety of theenvgene, and it appeared that most, but not all, of the SU gene product of FeLV-A, beginning from the N-terminus, can be replaced by sequences from an endogenous element, still allowing the virus to be biologically viable. In fact, these substitutions in theenvgene expanded infectivity of the parental FeLV-A from ecotropic to polytropic cell tropism. Additionally, substitutions in the SU region yielded many recombinants in which a primary neutralizing pentapeptide epitope of FeLV-A was altered because of its variance in the endogenous element. In several of the recombinants, this sequence was also found to be frequently mutated. Consistent with the changes identified in this antibody-binding domain, the recombinant viruses were only weakly inhibited by a monoclonal antibody directed against this epitope, while FeLV-A was highly sensitive to neutralization.
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猫基因组文库中内源性猫白血病病毒序列的分子克隆和表征
影响因子：
5.4
作者：
L. Soe;B. G. Devi;J. Mullins;Pradip ROY
通讯作者：
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影响因子：
64.8
作者：
J. Overbaugh;N. Riedel;E. Hoover;J. Mullins
通讯作者：
J. Mullins
影响因子：
--
作者：
Eric Hunter;R. Swanstrom
通讯作者：
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影响因子：
4.4
作者：
C. K. Grant;B. J. Ernisse;O. Jarrett;F. Jones
通讯作者：
F. Jones
影响因子：
56.9
作者：
HU, WS;TEMIN, HM
通讯作者：
TEMIN, HM