Identification, Mapping, and Molecular Marker Development for Rgsr8.1: A New Quantitative Trait Locus Conferring Resistance to Gibberella Stalk Rot in Maize (Zea mays L.).
Identification, Mapping, and Molecular Marker Development for Rgsr8.1: A New Quantitative Trait Locus Conferring Resistance to Gibberella Stalk Rot in Maize (Zea mays L.).
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DOI：
10.3389/fpls.2017.01355
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发表时间：
2017
影响因子：
5.6
通讯作者：
Yu GR
中科院分区：
文献类型：
--
作者：
Chen Q;Song J;Du WP;Xu LY;Jiang Y;Zhang J;Xiang XL;Yu GR
玉米秆腐病是世界范围内的一种主要真菌病害，化学方法难以防治。因此，在玉米育种中，赋予抗性的数量性状基因座（QTL）对于控制该病害非常重要。新一代测序技术被认为是建立农艺性状与分子标记或候选基因关联的快速有效的方法。在本研究中，我们采用 QTL-seq（一种基于全基因组重测序的方法）来识别赋予玉米茎腐病抗性的候选基因组区域。精细定位了一个新的抗性 QTL Rgsr8.1，赋予其对赤霉茎腐病 (GSR) 的广谱抗性。来自 18327（易感）和 S72356（抗性）杂交的 F2 和 BC1F1 群体的分离分析表明，对 GSR 的抗性可能是玉米的数量遗传性状。 QTL-seq结果显示，GSR抗性定位于8号染色体161.001至170.6 Mb。基于简单序列重复（SSR）标记、单核苷酸多态性（SNP）标记和重组测试，根据玉米参考基因组，Rgsr8.1的位置缩小到2.04 Mb，两侧是SSR-65和SNP-25标记，物理位置为164.69至166.72 Mb。在该区域中，发现了两个候选抗性基因，一个是生长素响应元件，另一个编码抗病蛋白。总之，这些结果将有助于玉米育种计划，以提高玉米对 GSR 的抗性。
Maize stalk rot is a major fungal disease worldwide, and is difficult to control by chemical methods. Therefore, in maize breeding, quantitative trait loci (QTLs) conferring resistance are important for controlling the disease. Next-generation sequencing technologies are considered a rapid and efficient method to establish the association of agronomic traits with molecular markers or candidate genes. In the present study, we employed QTL-seq, which is a whole-genome resequencing-based approach, to identify candidate genomic regions conferring resistance to maize stalk rot. A novel resistance QTL Rgsr8.1 was finely mapped, conferring broad-spectrum resistance to Gibberella stalk rot (GSR). Segregation analysis in F2 and BC1F1 populations, which were derived from a cross between 18327 (Susceptible) and S72356 (Resistant), indicated that the resistance to GSR was likely to be a quantitatively inherited trait in maize. The result of QTL-seq showed that the resistance to GSR was mapped on chromosome 8 from 161.001 to 170.6 Mb. Based on the simple sequence repeat (SSR) markers, single-nucleotide polymorphism (SNP) markers, and the recombinant test, the location of Rgsr8.1 was narrowed down to 2.04 Mb, flanked by SSR-65 and SNP-25 markers at the physical location from 164.69 to 166.72 Mb based on the maize reference genome. In this region, two candidate resistant genes were found with, one auxin-responsive elements and the other encoding a disease resistance protein. In summary, these results will be useful in maize breeding programs to improve the resistance to GSR in maize.
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影响因子：
14.9
作者：
GIOVANNONI, JJ;WING, RA;TANKSLEY, SD
通讯作者：
TANKSLEY, SD
影响因子：
4.9
作者：
Ahmad, Y;Hameed, A;Aslam, M
通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
Boddupalli, Prasanna M.
影响因子：
5.4
作者：
Foiada, Flavio;Westermeier, Peter;Schoen, Chris-Carolin
通讯作者：
Schoen, Chris-Carolin
影响因子：
3.1
作者：
Ding, Jun-Qiang;Wang, Xiao-Ming;Li, Jian-Sheng
通讯作者：
Li, Jian-Sheng