Immunomodulatory effect of Stichopus japonicus acid mucopolysaccharide on experimental hepatocellular carcinoma in rats.
Immunomodulatory effect of Stichopus japonicus acid mucopolysaccharide on experimental hepatocellular carcinoma in rats.
复制标题
海参酸性粘多糖对实验性肝癌大鼠的免疫调节作用
DOI：
10.3390/molecules18067179
复制
发表时间：
2013-06-19
期刊：
影响因子：
--
通讯作者：
Yang FG
中科院分区：
文献类型：
--
作者：
Song Y;Jin SJ;Cui LH;Ji XJ;Yang FG
海参酸粘多糖（StichopusAcidMucopolysaccharide，SJAMP）是从海参体壁中提取的一种重要的生物活性物质。本研究探讨了SJAMP在大鼠实验性肝细胞癌（HCC）模型中的抗肿瘤和免疫调节作用。向患有二乙基亚硝胺（DEN）诱导的肝癌的大鼠给予三剂SJAMP（17.5 mg/kg、35 mg/kg和70 mg/kg，每周5天经口灌胃给药）。SJAMP治疗通过减少结节的数量和平均体积，降低血清甲胎蛋白（AFP）水平和肝脏增殖细胞核抗原（PCNA）表达，以及增加p21表达，显著抑制DEN诱导的HCC。SJAMP还能降低血清ALT、AST、GGT和TNF-α水平，升高血清IL-2水平。SJAMP给药还改善了脾脏和胸腺功能指数，并改善了巨噬细胞吞噬作用和NK细胞介导的杀肿瘤活性。此外，CD 3+和CD 4 + T淋巴细胞水平显著恢复，CD 4 +/CD 8 + T细胞比率以剂量依赖性方式恢复正常。总之，SJAMP通过刺激免疫器官和组织增殖，有效抑制肝癌的生长，从而增强大鼠的细胞免疫途径。
Stichopus japonicus acid mucopolysaccharide (SJAMP) is an important biologically active compound that can be extracted from the body wall of the sea cucumber. The present study investigated the anti-tumor and immunomodulatory effects of SJAMP in an experimental hepatocellular carcinoma (HCC) model in rats. Three doses of SJAMP (17.5 mg/kg, 35 mg/kg, and 70 mg/kg administered 5 days/week via oral gavage) were given to rats with diethylnitrosamine (DEN)-induced HCC. SJAMP treatment significantly inhibited DEN-induced HCC by reducing both the number and mean volume of nodules, decreasing serum α-fetoprotein (AFP) levels and proliferating cell nuclear antigen (PCNA) expression in liver, and increasing p21 expression. Furthermore, SJAMP decreased the serum levels of ALT, AST, GGT and TNF-α and increased serum IL-2. SJAMP administration also improved indices of spleen and thymus function and improved both macrophage phagocytosis and NK cell-mediated tumoricidal activity. Moreover, CD3+ and CD4+ T lymphocyte levels recovered significantly and the CD4+/CD8+ T cell ratio normalized in a dose-dependent manner. In conclusion, SJAMP effectively inhibited the growth of HCC through the stimulation of immune organs and tissue proliferation, leading to the enhancement of cellular immunity pathways in rats.
登录
查看更多内容
影响因子：
3.1
作者：
Kariya, Y;Watabe, S;Ishii, T
通讯作者：
Ishii, T
影响因子：
5.4
作者：
Bordbar S;Anwar F;Saari N
通讯作者：
Saari N
影响因子：
20.3
作者：
Oflazoglu, Ezogelin;Stone, Ivan J.;Gerber, Hans-Peter
通讯作者：
Gerber, Hans-Peter
影响因子：
4.7
作者：
Oyama, K;Shiota, G;Kawasaki, H
通讯作者：
Kawasaki, H
影响因子：
9.7
作者：
Chen, GG;Lai, PBS;Lau, WY
通讯作者：
Lau, WY