Detection of human herpesviruses in cerebrospinal fluids collected from patients suspected of neuroinfectious diseases
Detection of human herpesviruses in cerebrospinal fluids collected from patients suspected of neuroinfectious diseases
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疑似神经传染病患者脑脊液中人类疱疹病毒的检测
DOI：
10.1007/s13365-021-01040-5
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发表时间：
2021
影响因子：
3.2
通讯作者：
Yoshikawa Tetsushi
中科院分区：
文献类型：
--
作者：
Ishimaru Soichiro;Kawamura Yoshiki;Miura Hiroki;Shima Sayuri;Ueda Akihiro;Watanabe Hirohisa;Mutoh Tatsuro;Yoshikawa Tetsushi
人类疱疹病毒（HHV）相关神经感染性疾病在免疫功能正常的成年人中的全谱仍不清楚。因此，我们试图阐明这些疾病的流行病学和临床特征。研究对象为2013年4月至2018年3月在我校医院接受神经科医生脊髓穿刺的16岁以上疑似神经感染性疾病患者。采用实时荧光定量PCR检测脑脊液中7种HHV dna的存在。322例患者中有33例（10.2%）检测到HHV dna。检出最多的疱疹病毒是水痘带状疱疹病毒（VZV）（19例），其次是HHV-6病毒（4例）、单纯疱疹病毒(HSV)-1病毒（3例）、HSV-2病毒（3例）和eb病毒（2例）。从脊髓炎、周围神经炎、脑炎和脑膜炎等各种神经感染性疾病患者收集的脑脊液中检测到HHV dna。所有携带1型单纯疱疹病毒DNA的患者都患有脑炎，而携带2型单纯疱疹病毒DNA的患者都患有脑膜炎。19名携带VZV DNA的患者中有11人患有脑膜炎。vzv相关脑炎患者（中位年龄80岁）明显大于非脑炎患者（中位年龄60.5岁）（P= 0.046）。13例无脑炎的VZV患者中有7例（54%）出现疱疹后神经痛，但4例脑炎患者未出现此类神经系统后遗症。总之，在这个队列中，hhv与大约10%的神经感染性疾病相关。VZV是最常见的病原体，可能是由于VZV脑膜炎患者人数众多。此外，与vzv相关的脑膜炎患者明显比与vzv相关的脑炎患者年轻。
The full spectrum of human herpesviruses (HHV)-associated neuroinfectious diseases in immunocompetent adults remains unclear. Hence, we sought to elucidate the epidemiology and clinical features of these diseases. The study subjects were patients over 16 years old suspected of neuroinfectious diseases who underwent spinal tap performed by neurologists in our university hospital between April 2013 and March 2018. The presence of seven HHV DNAs in cerebrospinal fluid (CSF) was determined by real-time PCR. HHV DNAs were detected in 33 (10.2%) of the 322 patients. The most frequently detected herpesvirus was varicella zoster virus (VZV) (19 patients), followed by HHV-6 (four patients), herpes simplex virus (HSV)-1 (three patients), HSV-2 (three patients), and Epstein-Barr virus (two patients). HHV DNAs were detected in CSF collected from patients with various neuroinfectious diseases, including myelitis, peripheral neuritis, encephalitis, and meningitis. All patients with HSV-1 DNA had encephalitis, whereas all patients with HSV-2 DNA had meningitis. Eleven of the 19 patients with VZV DNA had meningitis. Patients with VZV-associated encephalitis (median age, 80 years) were significantly older than non-encephalitis patients (median age, 60.5 years) (P= 0.046). Although post-herpetic neuralgia was observed in seven (54%) of the 13 patients with VZV and without encephalitis, no such neurological sequela was observed in the four encephalitis patients. In conclusion, HHVs were associated with approximately 10% of neuroinfectious diseases in this cohort. VZV was the most common pathogen, probably due to the large number of VZV meningitis patients. In addition, patients with VZV-associated meningitis were significantly younger than patients with VZV-associated encephalitis.
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DOI：
10.1086/593315
发表时间：
2008
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
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作者：
S. Troy;B. Blackburn;K. Yeom;A. F. Caulfield;M. Bhangoo;J. Montoya
通讯作者：
J. Montoya
影响因子：
9.9
作者：
Seeley, W. W.;Marty, F. M.;Bromfield, E. B.
通讯作者：
Bromfield, E. B.
影响因子：
4.8
作者：
Shiroshita, Kohei;Mori, Takehiko;Okamoto, Shinichiro
通讯作者：
Okamoto, Shinichiro
Tanaka N 等人：“在不相关的脐带血移植中监测四种疱疹病毒。”骨髓移植。
DOI：
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发表时间：
--
期刊：
影响因子：
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作者：
通讯作者：
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影响因子：
11.2
作者：
Fujino, Y;Nakajima, M;Yamada, T
通讯作者：
Yamada, T