Clostridium butyricum and Its Derived Extracellular Vesicles Modulate Gut Homeostasis and Ameliorate Acute Experimental Colitis.
Clostridium butyricum and Its Derived Extracellular Vesicles Modulate Gut Homeostasis and Ameliorate Acute Experimental Colitis.
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DOI：
10.1128/spectrum.01368-22
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发表时间：
2022-08-31
影响因子：
3.7
通讯作者：
Xiao, Yingping
中科院分区：
文献类型：
--
作者：
Ma, Lingyan;Shen, Qicheng;Lyu, Wentao;Lv, Lu;Wang, Wen;Yu, Minjie;Yang, Hua;Tao, Shiyu;Xiao, Yingping
预计微生物治疗将在未来炎症性肠病（IBD）的管理中发挥作用。丁酸梭菌（Clostridium butyricum，C.丁酸菌属）是一种益生菌微生物，其对各种疾病状况表现出有益作用。虽然许多研究表明C.丁酸杆菌对结肠炎小鼠有保护作用，C.丁酸菌在宿主中产生的有益结果仍不清楚。在这项研究中，我们研究了C。丁酸菌改变肠道微生物群，产生可能涉及的细菌代谢物，特别是微生物细胞外囊泡（EV）如何积极影响IBD，使用葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型。首先，我们证明了C。丁酸菌提供了对抗结肠炎的保护作用，如通过预防体重减轻、疾病活动指数（DAI）评分降低、结肠长度缩短、组织学评分降低和肠道屏障功能改善所证明的，伴随着病原菌（包括埃希氏菌/志贺氏菌）水平降低和产生丁酸盐的狭义梭菌-1和丁酸球菌的相对丰度增加。其次，我们还证实了肠道菌群和代谢产物产生的C。丁酸在DSS诱导的实验性结肠炎的减弱中起关键作用，这得到了粪便移植或由C.酪酸处理的小鼠。最后，C.丁酸衍生的EV保护了肠道屏障功能，改善了溃疡性结肠炎中的肠道微生物群稳态，并有助于整体结肠炎缓解。本研究表明，C.丁酸菌提供了针对结肠炎小鼠的预防效果，其涉及保护肠屏障和积极调节肠道微生物群。此外，我们证实了肠道菌群和代谢产物，诱导的C。丁酸也有助于DSS诱导的结肠炎的减弱。重要的是，C。酪酸衍生的EV在缓解结肠炎方面显示出有效的影响。
Microbiological treatments are expected to have a role in the future management of inflammatory bowel disease (IBD). Clostridium butyricum (C. butyricum) is a probiotic microorganism that exhibits beneficial effects on various disease conditions. Although many studies have revealed that C. butyricum provides protective effects in mice with colitis, the way C. butyricum establishes beneficial results in the host remains unclear. In this study, we investigated the mechanisms by which C. butyricum modifies the gut microbiota, produces bacterial metabolites that may be involved, and, specifically, how microbial extracellular vesicles (EVs) positively influence IBD, using a dextran sulfate sodium (DSS)-induced colitis murine model in mice. First, we showed that C. butyricum provides a protective effect against colitis, as evidenced by the prevention of body weight loss, a reduction in the disease activity index (DAI) score, a shortened colon length, decreased histology score, and an improved gut barrier function, accompanied by reduced levels of pathogenic bacteria, including Escherichia/Shigella, and an increased relative abundance of butyrate-producing Clostridium sensu stricto-1 and Butyricicoccus. Second, we also confirmed that the gut microbiota and metabolites produced by C. butyricum played key roles in the attenuation of DSS-induced experimental colitis, as supported by the profound alleviation of colitis effects following fecal transplantation or fecal filtrate insertion supplied from C. butyricum-treated mice. Finally, C. butyricum-derived EVs protected the gut barrier function, improved gut microbiota homeostasis in ulcerative colitis, and contributed to overall colitis alleviation. IMPORTANCE This study indicated that C. butyricum provided a prevention effect against colitis mice, which involved protection of the intestinal barrier and positively regulating gut microbiota. Furthermore, we confirmed that the gut microbiota and metabolites that were induced by C. butyricum also contributed to the attenuation of DSS-induced colitis. Importantly, C. butyricum-derived EVs showed an effective impact in alleviating colitis.
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影响因子：
6.4
作者：
Codemo M;Muschiol S;Iovino F;Nannapaneni P;Plant L;Wai SN;Henriques-Normark B
通讯作者：
Henriques-Normark B
影响因子：
7.3
作者：
Grondin JA;Kwon YH;Far PM;Haq S;Khan WI
通讯作者：
Khan WI
影响因子：
29.4
作者：
Ott, Stephan J.;Waetzig, Georg H.;Schreiber, Stefan
通讯作者：
Schreiber, Stefan
亚精胺可改善饮食诱导的肥胖小鼠的肠道屏障完整性和肠道微生物群功能。
影响因子：
12.2
作者：
Ma L;Ni Y;Wang Z;Tu W;Ni L;Zhuge F;Zheng A;Hu L;Zhao Y;Zheng L;Fu Z
通讯作者：
Fu Z
DOI：
10.1038/nrgastro.2017.88
发表时间：
2017-10
期刊：
Nature reviews. Gastroenterology & hepatology
影响因子：
--
作者：
Ni J;Wu GD;Albenberg L;Tomov VT
通讯作者：
Tomov VT