Distribution of Y-receptors in murine lingual epithelia.
Distribution of Y-receptors in murine lingual epithelia.
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DOI：
10.1371/journal.pone.0046358
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zolotukhin S
中科院分区：
文献类型：
--
作者：
Hurtado MD;Acosta A;Riveros PP;Baum BJ;Ukhanov K;Brown AR;Dotson CD;Herzog H;Zolotukhin S
神经肽Y(NPY)家族的多肽激素及其同源受体在许多组织中介导不同的生物学功能。最近，我们在小鼠和人类的唾液中发现了肠道饱满肽YY(PYY)，并确定了它在调节食物摄入量和维持体重中的作用。在这里，我们报告了所有NPY受体(Rs)、Y1R、Y2R、Y4R和Y5R在小鼠舌上皮中的表达模式的系统分析。应用免疫组织化学、原位杂交、免疫细胞化学和RT-PCR四种独立的方法，我们发现舌背背层角化复层上皮的不同形态层次表达Y受体的模式非常独特而又相互重叠。特别是，基础祖细胞的单层同时表达Y1和Y2受体。Y1Rs存在于基底旁棘细胞层和颗粒层，而分化的角质形成细胞表现为丰富的Y5Rs。Y4Rs在支配固有层和机械感受器的神经纤维中大量表达。Y2Rs阳性的基底上皮细胞通过增加细胞内钙离子对PYY3-36反应强烈，提示它们可能与唾液中的PYY发生功能上的相互作用。在环状乳突的味蕾中，一些味觉感受器细胞(TrCs)表达YRs，主要位于顶区，表明它们在味觉中潜在的作用。部分YR阳性的TRCs与神经细胞黏附分子(NCAM)共定位，提示这些TRCs可能与神经末梢有突触接触。综上所述，我们发现所有YRs在多种舌细胞类型中都有丰富的表达，包括上皮祖细胞、角质形成细胞、神经元树突和TrCs。这些结果表明，这些受体可能参与了多种功能的调节，包括增殖、分化、运动、味觉和饱腹感。
Peptide hormones and their cognate receptors belonging to neuropeptide Y (NPY) family mediate diverse biological functions in a number of tissues. Recently, we discovered the presence of the gut satiation peptide YY (PYY) in saliva of mice and humans and defined its role in the regulation of food intake and body weight maintenance. Here we report the systematic analysis of expression patterns of all NPY receptors (Rs), Y1R, Y2R, Y4R, and Y5R in lingual epithelia in mice. Using four independent assays, immunohistochemistry, in situ hybridization, immunocytochemistry and RT PCR, we show that the morphologically different layers of the keratinized stratified epithelium of the dorsal layer of the tongue express Y receptors in a very distinctive yet overlapping pattern. In particular, the monolayer of basal progenitor cells expresses both Y1 and Y2 receptors. Y1Rs are present in the parabasal prickle cell layer and the granular layer, while differentiated keratinocytes display abundant Y5Rs. Y4Rs are expressed substantially in the neuronal fibers innervating the lamina propria and mechanoreceptors. Basal epithelial cells positive for Y2Rs respond robustly to PYY3–36 by increasing intracellular Ca2+ suggesting their possible functional interaction with salivary PYY. In taste buds of the circumvallate papillae, some taste receptor cells (TRCs) express YRs localized primarily at the apical domain, indicative of their potential role in taste perception. Some of the YR-positive TRCs are co-localized with neuronal cell adhesion molecule (NCAM), suggesting that these TRCs may have synaptic contacts with nerve terminals. In summary, we show that all YRs are abundantly expressed in multiple lingual cell types, including epithelial progenitors, keratinocytes, neuronal dendrites and TRCs. These results suggest that these receptors may be involved in the mediation of a wide variety of functions, including proliferation, differentiation, motility, taste perception and satiation.
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