Immunoprotection of grass carp (Ctenopharyngodon idella) with recombinant interferon (rCiIFN) against GCHV infection
Immunoprotection of grass carp (Ctenopharyngodon idella) with recombinant interferon (rCiIFN) against GCHV infection
复制标题
重组干扰素 (rCiIFN) 对草鱼 (Ctenopharyngodon idella) 的 GCHV 感染的免疫保护作用
DOI：
10.1016/j.aquaculture.2013.01.017
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发表时间：
2013-04
期刊：
影响因子：
4.5
通讯作者：
Hu, Chengyu
中科院分区：
文献类型：
--
作者：
Li, Dongming;Lin, Gang;Yu, Xinjian;Huang, Shenghe;Lai, Qinan;Liu, Yong;Wu, Zhiqiang;Hu, Chengyu
研究了草鱼重组干扰素（rCiIFN）的免疫刺激作用和抗病毒活性。从大肠杆菌BL 21中表达并纯化了rCiIFN融合蛋白（约38 kD）。为了评价rCiIFN对草鱼出血症病毒（Grasscarp Hemorrhagic Virus，GCHV）感染的体内保护效力，我们在我们的实验中通过腹腔内（ip）注射和口服喂养施用它。在GCHV感染前24 h腹腔注射三种不同剂量的rCiIFN（0.1、1、10μg/g鱼体重）均提高了鱼的存活率。以1μg/g鱼体重注射的相对存活率（RPS）高于其他两种剂量。在GCHV感染前1、2和5天，每天一次用与rCiIFN混合的颗粒喂食鱼，剂量为1μg/g鱼体重。结果表明，连续投喂2 d组的RPS高于仅投喂1 d组和连续投喂5 d组。此外，我们观察到几个干扰素刺激的基因（ISG）大大上调。草鱼肾细胞（CIK）经rCiIFN刺激后，Mx和IFN基因的表达水平均显著增强，分别在6 h和12 h达到高峰。通过与PBS注射对照中观察到的基因表达水平比较，发现IFN、IRF 1、IRF 7、PKR、Mx和STAT 3的表达在rCiIFN注射的鱼的测试组织中显著上调，并且这些ISG中的大多数在处理后12小时内达到最高水平，并在72小时内下降。以1μ grCiIFN/g鱼体重的剂量，每天投喂1- 5d后，鱼体内IFN的表达量显著上调，投喂1 ~ 2d后，鱼体内IFN的表达量达到峰值。这些结果表明rCiIFN具有抗病毒活性，ip注射和口服rCiIFN均可有效保护草鱼免受GCHV感染。
The immunostimulatory effect and antiviral activity of grass carp (Ctenopharyngodon idella) recombinant interferon (rCiIFN) were investigated in the present study. The rCiIFN fusion protein (≈38 kD) was expressed and purified from BL21 Escherichia coli. To evaluate in vivo protective efficacy of rCiIFN against grass carp hemorrhagic virus (GCHV) infection, we administered it by intraperitoneal (ip) injection and oral feeding in our experiments. Intraperitoneal injections with three different rCiIFN dosages (0.1, 1, 10μg/g fish body weight) at 24h prior to GCHV infection all improved the survival rate of the fish. The relative percentage survival (RPS) from 1μg/g fish body weight injection was higher than those from other two dosages. Fish were fed with pellets mixed with rCiIFN at 1μg/g fish body weight once daily for 1, 2 and 5days before GCHV infection. The results indicated that the RPS from feeding for 2 consecutive days was higher than those from feeding for just 1 d or 5 consecutive days. Additionally, we observed that several IFN-stimulated genes (ISGs) were greatly up-regulated. After stimulation with rCiIFN, the expression levels of Mx and IFN genes in grass carp kidney cells (CIK) were highly enhanced and peaked within 6 and 12h, respectively. By comparison with gene expression levels observed in PBS-injected controls, expressions of IFN, IRF1, IRF7, PKR, Mx and STAT3 were found to be significantly up-regulated in tested tissues of rCiIFN-injected fish, and most of these ISGs reached the highest level within 12h post-treatment and declined within 72h. After feeding fish once daily for 1–5days with pellets mixed with rCiIFN at 1μg rCiIFN/g fish body weight, it was found that the expression level of IFN was significantly up-regulated and reached its peak level in tissues of those fish treated for 1 or 2days. These results demonstrated that rCiIFN had antiviral activity and ip injection as well as oral administration of rCiIFN was effective in protecting grass carp against GCHV infection.
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影响因子：
5.4
作者：
Altmann, SM;Mellon, MT;Kim, CH
通讯作者：
Kim, CH
DOI：
10.1089/jir.2006.26.256
发表时间：
2006-04
期刊：
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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