Differences in infrared spectroscopic data of connective tissues in transflectance and transmittance modes.
Differences in infrared spectroscopic data of connective tissues in transflectance and transmittance modes.
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DOI：
10.1016/j.aca.2013.03.053
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发表时间：
2013-05-24
影响因子：
6.2
通讯作者：
Pleshko N
中科院分区：
文献类型：
--
作者：
Hanifi A;McGoverin C;Ou YT;Safadi F;Spencer RG;Pleshko N
傅里叶变换红外成像光谱（FT-IRIS）已被广泛用于表征薄组织切片中大分子的组成和取向。早期和目前的研究正常和偏振FT-IRIS数据主要使用组织切片到红外透射基板，如作为盐窗口。最近，低发射率（“low-e”）基底的使用由于其低成本和有利的红外光学性质而变得非常受关注。然而，当使用低辐射载玻片时，数据是在透反射模式下收集的，而使用盐窗时，数据是在透射模式下收集的。在本研究中，我们研究了这两种模式评估结缔组织组成的可比性。FT-IRIS数据从软骨、骨和肌腱的连续切片和标准聚合物聚甲基丙烯酸甲酯中以透反射和透射模式获得。非偏振和偏振FTIR数据的吸光度不同，在某些情况下，峰位置不同，在透射和透射模式之间。然而，FT-IRIS分析的胶原纤维在软骨中的取向导致预期的带状排列的纤维在透射率和透反射率。我们的结论是，数值比较FT-IRIS衍生参数的组织成分应占基板类型和数据收集模式，而整体组织结构的分析模式之间可能更不变。
Fourier transform infrared imaging spectroscopy (FT-IRIS) has been used extensively to characterize the composition and orientation of macromolecules in thin tissue sections. Earlier and current studies of normal and polarized FT-IRIS data have primarily used tissues sectioned onto infrared transmissive substrates, such as salt windows. Recently, the use of low-emissivity (“low-e”) substrates has become of great interest because of their low cost and favourable infrared optical properties. However, data is collected in transflectance mode when using low-e slides and in transmittance mode using salt windows. In the current study we investigated the comparability of these two modes for assessment of the composition of connective tissues. FT-IRIS data were obtained in transflectance and transmittance modes from serial sections of cartilage, bone and tendon, and from a standard polymer, polymethylmethacrylate. Both non-polarized and polarized FTIR data differed in absorbance, and in some cases peak position, between transflectance and transmittance modes. However, the FT-IRIS analysis of the collagen fibril orientation in cartilage resulted in the expected zonal arrangement of fibrils in both transmittance and transflectance. We conclude that numerical comparison of FT-IRIS-derived parameters of tissue composition should account for substrate type and data collection mode, while analysis of overall tissue architecture may be more invariant between modes.
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影响因子：
11.2
作者：
GLASS, GV;PECKHAM, PD;SANDERS, JR
通讯作者：
SANDERS, JR
影响因子：
3.5
作者：
Kim, M;Bi, XH;Camacho, NP
通讯作者：
Camacho, NP
影响因子：
--
作者：
Barnes, RB;Bonner, LG
通讯作者：
Bonner, LG
影响因子：
4.2
作者：
ARSENAULT, AL;GRYNPAS, MD
通讯作者：
GRYNPAS, MD
影响因子：
3.3
作者：
Diem, M;Romeo, M;Lasch, P
通讯作者：
Lasch, P