Identification of four novel stu-miR169s and their target genes in Solanum tuberosum and expression profiles response to drought stress
Identification of four novel stu-miR169s and their target genes in Solanum tuberosum and expression profiles response to drought stress
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马铃薯中四种新型 Stu-miR169 及其靶基因的鉴定以及对干旱胁迫的表达谱响应
DOI：
10.1007/s00606-015-1242-x
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发表时间：
2015
影响因子：
1.9
通讯作者：
Wang Di
中科院分区：
文献类型：
--
作者：
Yang Jiangwei;Zhang Ning;Zhou Xiangyan;Si Huaijun;Wang Di
植物microrna是重要的内源基因调控因子，在转录后水平调控基因表达。先前的研究已经确定miR169家族成员调控NF-YA转录因子，这些转录因子与植物发育和胁迫反应有关。目前，报道的马铃薯基因组序列数据为全面了解马铃薯miR169/NF-YA模块的分子机制提供了机会。基于马铃薯基因组序列数据，预测了马铃薯中4个新的stu-miR169家族成员。miRNA靶标预测表明，成熟的stum - mir169序列在马铃薯StNF-YA基因的5个位点上有一个位点，其中3个位点通过RNA连接酶介导的5′race（5′RLM-RACE）试验得到验证。对成熟的stu-miR169及其预测靶基因表达谱的研究结果也表明，成熟的stu-miR169在干旱胁迫下表达下调。在干旱处理的不同时期，有一些StNF-YA靶向基因与成熟的stu-miR169表现出负表达模式。综上所述，stum - mir169的表达减少可能导致NF-YA家族成员的过度表达，这些成员与抗旱胁迫有关。
Plant microRNAs are important endogenous gene regulators which regulate gene expression at post-transcriptional level. Previous studies have identified that miR169 family members regulated the NF-YA transcription factors which have been implicated in plant development and stress responses. At present, reported potato genome sequence data offered an opportunity for global insights into the molecular mechanisms of the miR169/NF-YA modules in potato. In this work, 4 novel stu-miR169 family members were predicted in potato based on potato genome sequence data. miRNA target prediction showed that mature stu-miR169 sequences have a bite sit on the 5 of StNF-YA genes in potato, and three of them were validated by RNA ligase-mediated 5′RACE (5′ RLM-RACE) assay. The result from investigation of the expression patterns of mature stu-miR169 and their predicted target genes also showed that mature stu-miR169 was down-regulated in response to the drought stress. There were some targeted StNF-YA genes that exhibited a negative expression pattern with mature stu-miR169 during the different periods of drought-treated samples. Taken together, the decreased expression of stu-miR169 might drive over-expression of NF-YA family members, they are related to resistances against drought stress.
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