Multiple locus genealogies and phenotypic characters reappraise the causal agents of apple ring rot in China
Multiple locus genealogies and phenotypic characters reappraise the causal agents of apple ring rot in China
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多位点谱系和表型特征重新评估中国苹果环腐病的致病因子
DOI：
10.1007/s13225-014-0306-5
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发表时间：
2015-03
期刊：
影响因子：
20.3
通讯作者：
Sun Guangyu
中科院分区：
文献类型：
--
作者：
Xu Chao;Wang Chunsheng;Ju Liangliang;Zhang Rong;Biggs Alan R.;Tanaka Eiji;Li Bingzhi;Sun Guangyu
苹果环腐病给东亚苹果主产区造成严重的经济损失。该疾病的致病因子已被多种鉴定为Macrophoma kuwatsukai、Physalospora piricola 和Botryosphaeria berengerianaf。 sp.piricola，尽管B. dothidea是目前最广泛接受的病原体名称。分类学的不确定性推迟了有效管理这种破坏性疾病所需的研究。在本研究中，将谱系一致性系统发育物种识别（GCPSR）应用于来自中国多个地点的苹果和梨的致病真菌分离株以及几个参考分离株。基于四个核位点（ITS、EF-1α、HIS 和 HSP）序列的系统发育结果揭示了所检查分离株中存在两个物种。一种包括B的前表型分离株。 dothidea 和另一个包括先前指定为 B 的分离株。贝伦杰里亚夫。 sp.piricola。从形态学上看，后一个分类单元在 PDA 上呈现贴压的菌丝体垫，而 B. dothidea 展示了到达盖子的气生菌丝体柱，并且后一物种的分生孢子比 B. dothidea。在相对较高的温度下，Botryosphaeria dothidea 的生长速度比后者更快。致病性测试表明，后一类群在梨茎上引起大面积溃疡和水泡，而 B. dothide 是非致病性的，但在苹果芽上，这两种真菌分别引起大和小疣状突出物。总体而言，这个神秘物种与 B.B. 表现出足够的遗传变异和生物学差异。 dothidea。作为分类学研究的结果，我们在这里描述了后一个分类单元的新组合，Botryosphaeria kuwatsukai，并指定了表型。桑津凯和B. dothidea 被认为是中国和日本苹果环腐病的主要致病菌。
Apple ring rot inflicts severe economic losses in the main apple producing areas of East Asia. The causal agent of the disease has been variously identified asMacrophoma kuwatsukai,Physalospora piricolaandBotryosphaeria berengerianaf. sp.piricola, althoughB. dothideais currently the most widely accepted pathogen name. The taxonomic uncertainty has delayed research that is needed to manage effectively this destructive disease. In the present study, genealogical concordance phylogenetic species recognition (GCPSR) was applied to pathogenic fungal isolates from apple and pear from several locations in China, along with several reference isolates. Phylogenetic results based on sequences of four nuclear loci (ITS, EF-1α, HIS and HSP) revealed the existence of two species within the examined isolates. One includes an ex-epitype isolate ofB. dothideaand the other includes an isolate that was previously designated asB. berengerianaf. sp.piricola. Morphologically, the latter taxon presented an appressed mycelial mat on PDA whereasB. dothideadisplayed columns of aerial mycelia reaching the lids, and conidia of the latter species were longer thanB. dothidea. Botryosphaeria dothideahad a faster growth rate than the latter taxon under relatively high temperatures. Pathogenicity tests showed that on pear stems the latter taxon caused large-scale cankers along with blisters whereasB. dothideawas non-pathogenic, but on apple shoots the two fungi induced large and small wart-like prominences, respectively. Overall, this cryptic species demonstrated sufficient genetic variations and biological differences fromB. dothidea. As a result of taxonomic study, we described here the latter taxon in a new combination,Botryosphaeria kuwatsukaiand designate an epitype.BothB. kuwatsukaiandB. dothideaare considered to be the main causal agents for apple ring rot in China and Japan.
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影响因子：
20.3
作者：
Hyde, Kevin D.;Nilsson, R. Henrik;Zhou, Nan
通讯作者：
Zhou, Nan
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Guo Liyun
通讯作者：
Guo Liyun
DOI：
10.1111/j.1096-0031.1996.tb00196.x
发表时间：
1994-09-01
期刊：
CLADISTICS-THE INTERNATIONAL JOURNAL OF THE WILLI HENNIG SOCIETY
影响因子：
--
作者：
FARRIS, JS;KALLERSJO, M;BULT, C
通讯作者：
BULT, C
影响因子：
2.8
作者：
B. Slippers;G. Fourie;P. Crous;T. Coutinho;B. Wingfield;M. Wingfield
通讯作者：
B. Slippers;G. Fourie;P. Crous;T. Coutinho;B. Wingfield;M. Wingfield
DOI：
--
发表时间：
1984
期刊：
--
影响因子：
--
作者：
H. Koganezawa;T. Sakuma
通讯作者：
H. Koganezawa;T. Sakuma