Shared evolutionary origin of vertebrate neural crest and cranial placodes.
Shared evolutionary origin of vertebrate neural crest and cranial placodes.
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脊椎动物神经rest和颅骨位置的共同进化起源。
DOI：
10.1038/s41586-018-0385-7
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发表时间：
2018-08
期刊：
影响因子：
64.8
通讯作者：
Horie T
中科院分区：
文献类型：
--
作者：
Horie R;Hazbun A;Chen K;Cao C;Levine M;Horie T
介壳和神经嵴代表了脊椎动物的特征，尽管进行了广泛的研究，但它们之间的关系仍然不清楚。在这里，我们使用谱系追踪，基因破坏和单细胞RNA测序分析的组合，以探索的性质的侧板外胚层的原始脊椎动物，玻璃海鞘。在Ciona的侧板的图案和脊椎动物的神经板外胚层的区室化之间有明显的相似之处。这两个系统都表现出Six 1/2、Pax 3/7和Msxb表达的顺序模式，这些模式依赖于连锁调节相互作用的网络。在Ciona中，这种区室化网络产生不同但相关类型的感觉细胞，这些感觉细胞与脊椎动物中的颅基板和神经嵴的衍生物具有相似性。简单的遗传破坏导致一种感觉细胞类型转化为另一种。我们专注于双极尾神经元，因为它们来自侧板的尾部区域，具有背根神经节的特性，背根神经节是脊椎动物神经嵴的衍生物。值得注意的是，双极尾神经元很容易转化为触须感觉细胞，一个原基板的感觉细胞类型，从最前区的侧板在cionatadpole。通过全胚胎单细胞RNA测序测定证实了转化的证据。这些发现表明，侧板外胚层的区室化早于脊椎动物的出现，并作为颅基板和神经嵴的进化的共同来源。
Placodes and neural crests represent defining features of vertebrates, yet their relationship remains unclear despite extensive investigation, –. Here we use a combination of lineage tracing, gene disruption and single-cell RNA-sequencing assays to explore the properties of the lateral plate ectoderm of the proto-vertebrate,Ciona intestinalis. There are notable parallels between the patterning of the lateral plate inCionaand the compartmentalization of the neural plate ectoderm in vertebrates. Both systems exhibit sequential patterns ofSix1/2,Pax3/7andMsxbexpression that depend on a network of interlocking regulatory interactions. InCiona, this compartmentalization network produces distinct but related types of sensory cells that share similarities with derivatives of both cranial placodes and the neural crest in vertebrates. Simple genetic disruptions result in the conversion of one sensory cell type into another. We focused on bipolar tail neurons, because they arise from the tail regions of the lateral plate and possess properties of the dorsal root ganglia, a derivative of the neural crest in vertebrates. Notably, bipolar tail neurons were readily transformed into palp sensory cells, a proto-placodal sensory cell type that arises from the anterior-most regions of the lateral plate in theCionatadpole. Proof of transformation was confirmed by whole-embryo single-cell RNA-sequencing assays. These findings suggest that compartmentalization of the lateral plate ectoderm preceded the advent of vertebrates, and served as a common source for the evolution of both cranial placodes and neural crest,.
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