Toward Gene-Correlated Spatially Resolved Metabolomics with Fingerprint Coherent Raman Imaging.
Toward Gene-Correlated Spatially Resolved Metabolomics with Fingerprint Coherent Raman Imaging.
复制标题
DOI：
10.1021/acs.jpcb.3c01446
复制
发表时间：
2023-06-29
影响因子：
3.3
通讯作者：
Cicerone, Marcus T.
中科院分区：
文献类型：
--
作者：
Poorna, Rajas;Chen, Wei-Wen;Qiu, Peng;Cicerone, Marcus T.
人们早就知道，拉曼光谱可以提供足够的信息来区分不同的细胞表型。这种辨别能力的基础是拉曼光谱提供了随着转录活性变化的代谢谱的整体读数。将拉曼光谱的变化与特定信号通路的调节紧密地联系起来是可能的，但感兴趣的光谱信号可能很弱，并且在个体之间略有不同。因此，建立拉曼到转录组的映射将需要严格控制和易于操作的生物系统和高通量光谱获取。为了满足这些要求，我们尝试使用宽带相干反斯托克斯拉曼散射(BCARS)显微镜在亚细胞分辨率下对体内线虫两性性腺进行空间光谱映射。线虫两性性腺是一个理想的模型系统，具有高度调控的时空细胞事件的连续、连续过程。我们证明，BCARS的空间光谱特征与性腺中的基因表达谱相关，表明BCARS具有作为空间分辨组学替代物的潜力。
Raman spectroscopy has long been known to provide sufficient information to discriminate distinct cell phenotypes. Underlying this discriminating capability is that Raman spectra provide an overall readout of the metabolic profiles that change with transcriptomic activity. Robustly associating Raman spectral changes with the regulation of specific signaling pathways may be possible, but the spectral signals of interest may be weak and vary somewhat among individuals. Establishing a Raman-to-transcriptome mapping will thus require tightly controlled and easily manipulated biological systems and high-throughput spectral acquisition. We attempt to meet these requirements using broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to spatio-spectrally map the C. elegans hermaphrodite gonad in vivo at subcellular resolution. The C. elegans hermaphrodite gonad is an ideal model system with a sequential, continuous process of highly regulated spatiotemporal cellular events. We demonstrate that the BCARS spatio-spectral signatures correlate with gene expression profiles in the gonad, evincing that BCARS has potential as a spatially resolved omics surrogate.
登录
查看更多内容
影响因子：
2.8
作者：
Zahn, Jessica;Germond, Arno;Lundgren, Alice Y.;Cicerone, Marcus T.
通讯作者：
Cicerone, Marcus T.
影响因子：
35
作者：
通讯作者：
--
影响因子：
3.5
作者：
Matthews, Q.;Brolo, A. G.;Jirasek, A.
通讯作者：
Jirasek, A.
影响因子：
2.7
作者：
HIRSH, D;OPPENHEIM, D;KLASS, M
通讯作者：
KLASS, M
影响因子：
3.5
作者：
Chen, Szu-Yu;Hsieh, Cho-Shuen;Sun, Chi-Kuang
通讯作者：
Sun, Chi-Kuang