Surface charge-dependent mitochondrial response to similar intracellular nanoparticle contents at sublethal dosages.
Surface charge-dependent mitochondrial response to similar intracellular nanoparticle contents at sublethal dosages.
复制标题
亚致死剂量下，线粒体对相似的细胞内纳米颗粒内容物的表面电荷依赖性反应。
DOI：
10.1186/s12989-021-00429-8
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发表时间：
2021-09-26
影响因子：
10
通讯作者：
Jiang G
中科院分区：
文献类型：
--
作者：
Jin X;Yu H;Zhang Z;Cui T;Wu Q;Liu X;Gao J;Zhao X;Shi J;Qu G;Jiang G
考虑到人类频繁接触纳米颗粒的必然性，了解纳米颗粒的生物安全性对于合理使用纳米颗粒具有重要意义。巨噬细胞作为先天免疫系统的重要组成部分，广泛分布于重要组织中，也是吞噬颗粒的优势细胞类型。线粒体是巨噬细胞接触NPs时最敏感的细胞器之一。然而，以往的研究主要报道了大剂量NP治疗后线粒体的反应。在这里，我们以金纳米颗粒(AuNPs)为模型，研究了亚致死浓度的NPs对线粒体的影响。在相同的内照射剂量下，不同的AuNPs对线粒体的改变表现出不同程度的影响，包括管状线粒体减少，线粒体受损，活性氧增加，三磷酸腺苷减少。聚类分析、双因素方差分析和多元线性回归分析表明，AuNPs的表面性质是线粒体反应的主要决定因素。相关分析表明，随着Zeta电位由负变正，线粒体反应增强。线粒体呼吸链蛋白的变化表明，复合体V是线粒体对低剂量NPs反应的指标。我们的研究表明，即使在亚致死剂量下，修饰的AuNPs对线粒体的潜在危害，表明表面修饰在改善生物相容性方面的可能性，并为更好地评价纳米材料的生物安全性提供了一种新的途径。网上版载有补充材料，可在10.1186/s12989-021-00429-8查阅。
Considering the inevitability for humans to be frequently exposed to nanoparticles (NPs), understanding the biosafety of NPs is important for rational usage. As an important part of the innate immune system, macrophages are widely distributed in vital tissues and are also a dominant cell type that engulfs particles. Mitochondria are one of the most sensitive organelles when macrophages are exposed to NPs. However, previous studies have mainly reported the mitochondrial response upon high-dose NP treatment. Herein, with gold nanoparticles (AuNPs) as a model, we investigated the mitochondrial alterations induced by NPs at a sublethal concentration. At a similar internal exposure dose, different AuNPs showed distinct degrees of effects on mitochondrial alterations, including reduced tubular mitochondria, damaged mitochondria, increased reactive oxygen species, and decreased adenosine triphosphate. Cluster analysis, two-way ANOVA, and multiple linear regression suggested that the surface properties of AuNPs were the dominant determinants of the mitochondrial response. Based on the correlation analysis, the mitochondrial response was increased with the change in zeta potential from negative to positive. The alterations in mitochondrial respiratory chain proteins indicated that complex V was an indicator of the mitochondrial response to low-dose NPs. Our current study suggests potential hazards of modified AuNPs on mitochondria even under sublethal dose, indicates the possibility of surface modification in biocompatibility improvement, and provides a new way to better evaluation of nanomaterials biosafety. The online version contains supplementary material available at 10.1186/s12989-021-00429-8.
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影响因子：
46.2
作者：
Behzadi S;Serpooshan V;Tao W;Hamaly MA;Alkawareek MY;Dreaden EC;Brown D;Alkilany AM;Farokhzad OC;Mahmoudi M
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影响因子：
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Liu, Xiangsheng;Huang, Nan;Ji, Jian
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影响因子：
17.1
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通讯作者：
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影响因子：
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通讯作者：
Caruso, Giuseppe
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影响因子：
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作者：
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通讯作者：
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