Genome-wide association study of meat quality traits in a White Duroc×Erhualian F2 intercross and Chinese Sutai pigs.
Genome-wide association study of meat quality traits in a White Duroc×Erhualian F2 intercross and Chinese Sutai pigs.
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白杜洛克×二花莲F2杂交与中国苏泰猪肉质性状的全基因组关联研究
DOI：
10.1371/journal.pone.0064047
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Ma J;Yang J;Zhou L;Zhang Z;Ma H;Xie X;Zhang F;Xiong X;Cui L;Yang H;Liu X;Duan Y;Xiao S;Ai H;Ren J;Huang L
连锁作图研究已经发现了数以千计的肉质性状QTL，但大多数QTL在群体间缺乏精确定位或重复性，阻碍了其在猪育种中的应用。为了将影响肉质性状的QTL定位到精确的基因组区域，本研究利用Illumina PorcineSNP 60 K微珠芯片对两个猪群体（434头苏太猪和933头白色杜洛克×二花脸杂交F2代猪）进行了全基因组关联（GWA）研究。结果表明，在肉品质方面，马肉的pH值、色泽、滴水损失、水分含量、蛋白质含量、肌内脂肪含量、大理石花纹和硬度等指标均优于马肉。longissimus（LM）和M.半膜肌（SM）的肌肉，记录在两个种群。总共鉴定了这些性状的127个染色体范围内显著的SNP。其中，11个SNPs达到基因组显著性水平，包括1个在SSC3上的pH，1个在SSC3上的滴水损失和3个在SSC15上的滴水损失，3个（未定位）颜色a*，以及2个在SSC9和SSCX上的IMF。除11个未定位的SNPs外，116个显著的SNPs分布在28个约10 Mb或更小的基因组区域内，这些区域大多与先前报道的QTL区域相对应，并且跨越的区间比以前更小。SSC3和SSC7上的基因座对几个相关性状具有多效效应。除此之外，还有一些QTL信号在两个群体间重复。此外，我们确定了13个新的候选基因IMF，大理石花纹和坚定性，根据他们的位置，功能注释和报告的表达模式。这些结果将有助于进一步确定这些QTL背后的因果突变和未来的标记辅助选择在猪。
Thousands of QTLs for meat quality traits have been identified by linkage mapping studies, but most of them lack precise position or replication between populations, which hinder their application in pig breeding programs. To localize QTLs for meat quality traits to precise genomic regions, we performed a genome-wide association (GWA) study using the Illumina PorcineSNP60K Beadchip in two swine populations: 434 Sutai pigs and 933 F2 pigs from a White Duroc×Erhualian intercross. Meat quality traits, including pH, color, drip loss, moisture content, protein content and intramuscular fat content (IMF), marbling and firmness scores in the M. longissimus (LM) and M. semimembranosus (SM) muscles, were recorded on the two populations. In total, 127 chromosome-wide significant SNPs for these traits were identified. Among them, 11 SNPs reached genome-wise significance level, including 1 on SSC3 for pH, 1 on SSC3 and 3 on SSC15 for drip loss, 3 (unmapped) for color a*, and 2 for IMF each on SSC9 and SSCX. Except for 11 unmapped SNPs, 116 significant SNPs fell into 28 genomic regions of approximately 10 Mb or less. Most of these regions corresponded to previously reported QTL regions and spanned smaller intervals than before. The loci on SSC3 and SSC7 appeared to have pleiotropic effects on several related traits. Besides them, a few QTL signals were replicated between the two populations. Further, we identified thirteen new candidate genes for IMF, marbling and firmness, on the basis of their positions, functional annotations and reported expression patterns. The findings will contribute to further identification of the causal mutation underlying these QTLs and future marker-assisted selection in pigs.
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影响因子：
2.9
作者：
Cherel P;Pires J;Glénisson J;Milan D;Iannuccelli N;Hérault F;Damon M;Le Roy P
通讯作者：
Le Roy P
影响因子：
4.4
作者：
Grindflek E;Lien S;Hamland H;Hansen MH;Kent M;van Son M;Meuwissen TH
通讯作者：
Meuwissen TH
影响因子：
4.3
作者：
Bowers, Robert R.;Lane, M. Daniel
通讯作者：
Lane, M. Daniel
影响因子：
--
作者：
Iiritano, Stefania;Chiefari, Eusebio;Brunetti, Antonio
通讯作者：
Brunetti, Antonio
影响因子：
4.4
作者：
Gregersen VR;Conley LN;Sørensen KK;Guldbrandtsen B;Velander IH;Bendixen C
通讯作者：
Bendixen C