Large scale genome-wide association and LDLA mapping study identifies QTLs for boar taint and related sex steroids.
Large scale genome-wide association and LDLA mapping study identifies QTLs for boar taint and related sex steroids.
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DOI：
10.1186/1471-2164-12-362
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发表时间：
2011-07-13
期刊：
影响因子：
4.4
通讯作者：
Meuwissen TH
中科院分区：
文献类型：
--
作者：
Grindflek E;Lien S;Hamland H;Hansen MH;Kent M;van Son M;Meuwissen TH
在大量未阉割的公猪中观察到公猪异味，其特征是熟肉中具有令人不快的气味/味道，这主要是由雄烯酮和粪臭素水平升高引起的。雄烯酮是睾丸中产生的类固醇，与睾酮和雌激素等其他性类固醇的生物合成同时进行。当在育种计划中针对雄烯酮进行选择而不同时降低其他类固醇的水平时，这是一个挑战。本研究的目的是利用高密度全基因组关联 (GWA) 与连锁不平衡连锁分析 (LDLA) 相结合，在商业长白猪 (n = 1,251) 和杜洛克 (n = 918) 品种中鉴定与公猪异味化合物和相关性类固醇相关的数量性状位点 (QTL)。总而言之，皮下脂肪中雄烯酮的 14 个全基因组显着性 (GWS) QTL 区域是从长白猪的 LDLA 研究中获得的，以及杜洛克猪中的 14 个 GWS QTL 区域。 LDLA分析显示，两个品种均获得了其中7个QTL区域，分别位于SSC 1、2、3、7和15上。长白品种中的所有 14 个 GWS 雄烯酮 QTL 也在染色体显着性 (CWS) 或 GWS 水平上影响雌激素，而在杜洛克中，14 个 QTL 中的 3 个影响雄烯酮而不影响任何雌激素。对于粪臭素，长白猪和杜洛克的 LDLA 分析中分别有 10 个和 4 个 QTL 是 GWS，其中 4 个 QTL 在两个品种中均检测到。通过 LDLA 获得的粪臭素的 GWS QTL 位于 SSC 1、5、6、7、10、11、13 和 14。这是第一份应用猪 60 K SNP 阵列同时分析公猪异味化合物和相关性激素的报告，同时使用 GWA 和 LDLA 方法。一些QTL参与雄烯酮和粪臭素的调节，并且大多数雄烯酮QTL也影响雌激素水平。尽管大多数 QTL 是品种特异性的，但在一个品种中检测到了 7 个雄烯酮 QTL，并在另一个品种（即在独立样本中）中得到了确认。大多数粪臭素 QTL 不会对其他性激素产生负面影响，并且应该更容易在育种计划中实施。
Boar taint is observed in a high proportion of uncastrated male pigs and is characterized by an unpleasant odor/flavor in cooked meat, primarily caused by elevated levels of androstenone and skatole. Androstenone is a steroid produced in the testis in parallel with biosynthesis of other sex steroids like testosterone and estrogens. This represents a challenge when performing selection against androstenone in breeding programs, without simultaneously decreasing levels of other steroids. The aim of this study was to use high-density genome wide association (GWA) in combination with linkage disequilibrium-linkage analysis (LDLA) to identify quantitative trait loci (QTL) associated with boar taint compounds and related sex steroids in commercial Landrace (n = 1,251) and Duroc (n = 918) breeds. Altogether, 14 genome wide significant (GWS) QTL regions for androstenone in subcutaneous fat were obtained from the LDLA study in Landrace and 14 GWS QTL regions in Duroc. LDLA analysis revealed that 7 of these QTL regions, located on SSC 1, 2, 3, 7 and 15, were obtained in both breeds. All 14 GWS androstenone QTLs in Landrace are also affecting the estrogens at chromosome wise significance (CWS) or GWS levels, while in Duroc, 3 of the 14 QTLs affect androstenone without affecting any of the estrogens. For skatole, 10 and 4 QTLs were GWS in the LDLA analysis for Landrace and Duroc respectively, with 4 of these detected in both breeds. The GWS QTLs for skatole obtained by LDLA are located at SSC 1, 5, 6, 7, 10, 11, 13 and 14. This is the first report applying the Porcine 60 K SNP array for simultaneous analysis of boar taint compounds and related sex hormones, using both GWA and LDLA approaches. Several QTLs are involved in regulation of androstenone and skatole, and most of the QTLs for androstenone are also affecting the levels of estrogens. Seven QTLs for androstenone were detected in one breed and confirmed in the other, i.e. in an independent sample, although the majority of QTLs are breed specific. Most QTLs for skatole do not negatively affect other sex hormones and should be easier to implement into the breeding scheme.
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影响因子：
2.9
作者：
Duijvesteijn N;Knol EF;Merks JW;Crooijmans RP;Groenen MA;Bovenhuis H;Harlizius B
通讯作者：
Harlizius B
影响因子：
3.3
作者：
Grindflek, E.;Meuwissen, T. H. E.;Lien, S.
通讯作者：
Lien, S.
影响因子：
2.9
作者：
Grindflek E;Berget I;Moe M;Oeth P;Lien S
通讯作者：
Lien S
影响因子：
4.8
作者：
Fan, Jinjiang;Traore, Kassim;Papadopoulos, Vassilios
通讯作者：
Papadopoulos, Vassilios
影响因子：
3.8
作者：
Diaz, GJ;Squires, EJ
通讯作者：
Squires, EJ