Relationships between pond water and tilapia skin microbiomes in aquaculture ponds in Malawi
Relationships between pond water and tilapia skin microbiomes in aquaculture ponds in Malawi
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马拉维水产养殖池塘池塘水与罗非鱼皮肤微生物组之间的关系
DOI：
10.1101/2021.12.06.470702
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
McMurtrie J
中科院分区：
文献类型：
--
作者：
McMurtrie J
为了支持快速增长的全球人口，特别是中低收入国家的人口，对粮食产量的需求不断增加，推动了鱼类养殖实践的强化。然而，生产的密集化增加了疾病爆发的风险，从而增加了作物损失的可能性。鱼类皮肤粘膜表面的微生物群落知之甚少，但对维持鱼类健康和抗病能力很重要。这种皮肤微生物群落很容易受到与运输、处理和密集实践环境相关的压力因素的破坏，这有可能导致致病病原体的传播。在这项研究中，我们表征了罗非鱼皮肤上发现的微生物组合-全球养殖最广泛的有鳍鱼类-以及马拉维不同地理区域两个池塘系统中七个土制水产养殖池塘周围的水。元条形码方法被用来对原核和微真核生物群落进行测序。我们发现92%的原核扩增子序列变异体在皮肤和水样本中是共同的。不同的丰富和核心类群，但是，不同的皮肤和水样本。在罗非鱼皮中，富集了Cetobacterium、Paucibacter、Pseudomonas和Comamonadaceae，而在池塘水中，蓝藻Cyanobium、Microcystis和/或Synechocystis和CycloCyclotella是最普遍的。池塘，聚集在一起，根据他们的水原核生物群落也有类似的microeukaryotic社区，原核生物和microeukaryotic社区结构的强烈环境影响。虽然在池塘水中观察到强烈的位点特异性聚类，但罗非鱼皮肤原核生物的池塘位点分组不太明显，表明鱼类微生物群对环境影响具有更大的缓冲能力。与马拉维罗非鱼养殖相关的微生物群落的特征多样性，结构和变异为研究未来强化措施如何导致微生物生态失调和疾病发作提供了基线。
Intensification of fish farming practices is being driven by the demand for increased food production to support a rapidly growing global human population, particularly in lower-middle income countries. Intensification of production, however, increases the risk of disease outbreaks and thus the likelihood for crop losses. The microbial communities that colonise the skin mucosal surface of fish are poorly understood, but are important in maintaining fish health and resistance against disease. This skin microbial community is susceptible to disruption through stressors associated with transport, handling and the environment of intensive practices, and this risks the propagation of disease-causing pathogens. In this study, we characterised the microbial assemblages found on tilapia skin — the most widely farmed finfish globally — and in the surrounding water of seven earthen aquaculture ponds from two pond systems in distinct geographic regions in Malawi. Metabarcoding approaches were used to sequence the prokaryotic and microeukaryotic communities. We found 92% of prokaryotic amplicon sequence variants were common to both skin and water samples. Differentially enriched and core taxa, however, differed between the skin and water samples. In tilapia skin,Cetobacterium,Paucibacter,Pseudomonasand Comamonadaceae were enriched, whereas, the cyanobacteriaCyanobium,Microcystisand/orSynechocystis, and the diatomCyclotella, were most prevalent in pond water. Ponds that clustered together according to their water prokaryotic communities also had similar microeukaryotic communities indicating strong environmental influences on prokaryotic and microeukaryotic community structures. While strong site-specific clustering was observed in pond water, the grouping of tilapia skin prokaryotes by pond site was less distinct, suggesting fish microbiota have a greater buffering capacity against environmental influences. The characterised diversity, structure and variance of microbial communities associated with tilapia culture in Malawi provide the baseline for studies on how future intensification practices may lead to microbial dysbiosis and disease onset.
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DOI：
10.3390/v13071291
发表时间：
2021-07-02
期刊：
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影响因子：
--
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