Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
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DOI：
10.1099/jgv.0.000763
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Kaiser P
中科院分区：
文献类型：
--
作者：
Chakraborty P;Vervelde L;Dalziel RG;Wasson PS;Nair V;Dutia BM;Kaiser P
Marek病毒病毒（MDV）是一种α鞘内病毒，可在体内诱导T细胞淋巴瘤。 B细胞和活化的T细胞可以在B和T细胞中检测到体内的T细胞和巨噬细胞，但可以在体外感染B和T细胞在体外感染的巨噬细胞尚未成功。 Chicken CSF-1或Chicken IL-4和Chicken CSF-2分别产生巨噬细胞和DC，然后与FACS分类的鸡肉胚胎成纤维细胞（CEF）共同培养用EGFP表达和吞噬细胞特异性mAb鉴定出感染EGFP的重组MDV。 -PCR在感染的吞噬细胞中提供了MDV复制的证据与CEF的感染巨噬细胞的共同培养表明，在这些细胞类型中可能会发生生产性病毒感染。
Marek’s disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells. Chicken bone marrow cells were cultured with chicken CSF-1 or chicken IL-4 and chicken CSF-2 for 4 days to produce macrophages and DCs, respectively, and then co-cultured with FACS-sorted chicken embryo fibroblasts (CEFs) infected with recombinant MDV expressing EGFP. Infected phagocytes were identified and sorted by FACS using EGFP expression and phagocyte-specific mAbs. Detection of MDV-specific transcripts of ICP4 (immediate early), pp38 (early), gB (late) and Meq by RT-PCR provided evidence for MDV replication in the infected phagocytes. Time-lapse confocal microscopy was also used to demonstrate MDV spread in these cells. Subsequent co-culture of infected macrophages with CEFs suggests that productive virus infection may occur in these cell types. This is the first report of in vitro infection of phagocytic cells by MDV.
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