The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization.
The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization.
复制标题
CdSe/ZnS量子点对体内卵巢功能和体外受精的生殖毒性
DOI：
10.1038/srep37677
复制
发表时间：
2016-11-23
影响因子：
4.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Xu G;Lin G;Lin S;Wu N;Deng Y;Feng G;Chen Q;Qu J;Chen D;Chen S;Niu H;Mei S;Yong KT;Wang X
尽管量子点（QD）在生物医学和光电上有用，但它们的毒性风险仍然是临床使用的主要障碍，因此我们研究了CDSE/ZNS QD的生殖毒性在两个方面，（I） ii）体外受精过程。然后，分配了卵巢蛋白酶受体（FSHR）和黄体雌酮受体（LHR）结果表明，在卵巢中发现了QD，但在雌性小鼠上没有检测到的行为和发情周期。当QDS剂量高于1.0 pmol/天，我们发现QD的存在降低了QDS的情况下，卵母细胞显着下降。会对卵巢功能造成不利影响，并且此类QD在临床使用中的应用可能有限。
Despite the usefulness of quantum dots (QDs) in biomedicine and optoelectronics, their toxicity risks remain a major obstacle for clinical usages. Hence, we studied the reproductive toxicity of CdSe/ZnS QDs on two aspects, (i) in vivo ovarian functions and (ii) in vitro fertilization process. The body weight, estrous cycles, biodistribution of QDs, and oocyte maturation are evaluated on female mice treated with QDs. The mRNA level of the follicle-stimulating hormone receptor (FSHr) and luteinizing hormone receptor (LHr) in ovaries are assayed. Then, the matured cumulus-oocyte-complexes are harvested to co-culture with in vitro capacitated sperms, and the in vitro fertilization is performed. The result revealed that QDs are found in the ovaries, but no changes are detected on the behavior and estrous cycle on the female mice. The mRNA downregulations of FSHr and LHr are observed and the number of matured oocytes has shown a significant decrease when the QDs dosage was above 1.0 pmol/day. Additionally, we found the presence of QDs has reduced the in vitro fertilization success rate. This study highly suggests that the exposure of CdSe/ZnS QDs to female mice can cause adverse effects to the ovary functions and such QDs may have limited applications in clinical usage.
登录
查看更多内容
影响因子：
5.5
作者：
Jain, Manasi P.;Vaisheva, Farida;Maysinger, Dusica
通讯作者：
Maysinger, Dusica
影响因子：
3.6
作者：
OShaughnessy, PJ;McLelland, D;McBride, MW
通讯作者：
McBride, MW
影响因子：
2.7
作者：
Sun, FY;Betzendahl, I;Eichenlaub-Ritter, U
通讯作者：
Eichenlaub-Ritter, U
影响因子：
10.4
作者：
Geys J;Nemmar A;Verbeken E;Smolders E;Ratoi M;Hoylaerts MF;Nemery B;Hoet PH
通讯作者：
Hoet PH
影响因子：
10.2
作者：
Feugang JM;Youngblood RC;Greene JM;Fahad AS;Monroe WA;Willard ST;Ryan PL
通讯作者：
Ryan PL