An AFM Approach Applied in a Study of α-Crystallin Membrane Association: New Insights into Lens Hardening and Presbyopia Development.
An AFM Approach Applied in a Study of α-Crystallin Membrane Association: New Insights into Lens Hardening and Presbyopia Development.
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应用AFM方法研究α-晶状体蛋白膜缔合：对透镜硬化和老视发展的新见解。
DOI：
10.3390/membranes12050522
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发表时间：
2022-05-14
期刊：
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
--
作者：
随着年龄的增长，晶状体失去弹性，而α-晶体蛋白与晶状体膜的结合随着年龄的增长而增加。α-晶体蛋白与晶状体膜的结合与晶状体弹性丧失之间是否存在相关性尚不清楚。本研究首次利用原子力显微镜（AFM）研究了α-晶体蛋白膜的结合，研究了α-晶体蛋白膜的形貌图像和力学性能（突破力和膜面积压缩模量（KA）作为弹性指标）。从牛晶状体皮层中提取α-晶体蛋白，与在云母平面上制备的支撑脂质膜（SLM）孵育。AFM图像显示α-晶体蛋白与SLM的相互作用具有时间依赖性。力谱分析结果显示，在没有α-晶体蛋白结合的膜区得到的力曲线中存在突破事件，表明膜的弹性得到了保持。在膜中α-晶体蛋白浸没区和靠近α-晶体蛋白结合区的力曲线在定义的峰值力阈值内没有出现突破事件，表明膜失去弹性。我们的研究结果表明，α-晶体蛋白与膜的结合使膜弹性恶化，为理解晶状体硬化和老花眼的分子基础提供了新的见解。
The lens of the eye loses elasticity with age, while α-crystallin association with the lens membrane increases with age. It is unclear whether there is any correlation between α-crystallin association with the lens membrane and loss in lens elasticity. This research investigated α-crystallin membrane association using atomic force microscopy (AFM) for the first time to study topographical images and mechanical properties (breakthrough force and membrane area compressibility modulus (KA), as measures of elasticity) of the membrane. α-Crystallin extracted from the bovine lens cortex was incubated with a supported lipid membrane (SLM) prepared on a flat mica surface. The AFM images showed the time-dependent interaction of α-crystallin with the SLM. Force spectroscopy revealed the presence of breakthrough events in the force curves obtained in the membrane regions where no α-crystallin was associated, which suggests that the membrane’s elasticity was maintained. The force curves in the α-crystallin submerged region and the close vicinity of the α-crystallin associated region in the membrane showed no breakthrough event within the defined peak force threshold, indicating loss of membrane elasticity. Our results showed that the association of α-crystallin with the membrane deteriorates membrane elasticity, providing new insights into understanding the molecular basis of lens hardening and presbyopia.
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影响因子：
3.4
作者：
Heys, Karl R.;Truscott, Roger J. W.
通讯作者：
Truscott, Roger J. W.
DOI：
10.1016/bs.pmbts.2015.04.006
发表时间：
2015
影响因子：
--
作者：
Hejtmancik JF;Shiels A
通讯作者：
Shiels A
影响因子：
5.6
作者：
Domingues MM;Gomes B;Hollmann A;Santos NC
通讯作者：
Santos NC
影响因子：
2.4
作者：
Das, Chinmay;Sheikh, Khizar H.;Connell, Simon D.
通讯作者：
Connell, Simon D.
影响因子：
2
作者：
IFEANYI, F;TAKEMOTO, L
通讯作者：
TAKEMOTO, L