Bovine lactotroph cultures for the study of prolactin synthesis functions
Bovine lactotroph cultures for the study of prolactin synthesis functions
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用于研究催乳素合成功能的牛催乳素培养物
DOI：
10.1007/s11626-015-9974-z
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Juxiong Liu
中科院分区：
文献类型：
--
作者：
Jianfa Wang;Zhanqing Yang;Shoupeng Fu;Bingrun Liu;Dianjun Wu;Wei Wang;Dongbo Sun;Rui Wu;Juxiong Liu
本研究旨在建立体外表达催乳素（PRL）的牛垂体前叶源性促乳细胞（BAPDL），以研究其合成和分泌PRL的机制。新生犊牛垂体前叶PRL免疫组化染色结果表明，泌乳素细胞主要位于垂体前叶外侧翼的上级缘。将垂体前叶侧翼上级缘组织分散并在添加10%胎牛血清（FBS）的Dulbecco改良Eagle培养基（DMEM）中培养。采用有限稀释法从单细胞克隆中建立BAPDL。BAPDL细胞持续表达PRL和垂体特异性转录因子1（Pit-1）基因，并在添加10%FBS的DMEM中稳定快速生长。第20代BAPDL中存在PRL免疫反应性。第20代BAPDL培养上清中的牛PRL浓度比第1代BAPDL中的牛PRL浓度降低至35%以下。观察人表皮生长因子（hEGF）和多巴胺（DA）对第4代BAPDL细胞PRL表达和分泌的影响。这一结果与以往的研究结果一致。因此，它可以成功地用于研究刺激调节PRL合成和释放的机制。
The aim of this study was to establish a bovine anterior pituitary-derived lactotroph (BAPDL) line that expresses prolactin (PRL) in vitro to study the mechanisms of bovine PRL synthesis and secretion. Immunohistochemistry assay of PRL in the newborn calves’ anterior pituitary glands showed that most lactotrophs were located within the superior border of the lateral wings of the anterior pituitary. Tissues of the superior border of the lateral wings of the anterior pituitary were dispersed and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The limiting dilution method was used to establish BAPDL from single cell clone. BAPDL cells constantly expressed mRNAs for PRL and pituitary-specific transcription factor 1 (Pit-1) gene and grew steadily and rapidly in the DMEM supplemented with 10% FBS. PRL immunoreactivity was present in BAPDL at passage 20. The concentration of bovine PRL in BAPDL at passage 20 culture supernatant was decreased to below 35% compared with that in BAPDL at passage 1. The effects of human epidermal growth factor (hEGF) and dopamine (DA) on the expression and secretion of PRL in BAPDL at passage 4 were also investigated. The results are consistent with those of previous studies. Thus, it can be used successfully for studying the mechanisms of stimuli regulating PRL synthesis and release.
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影响因子：
20.3
作者：
N. Ben-Jonathan;R. Hnasko
通讯作者：
N. Ben-Jonathan;R. Hnasko
影响因子：
3.2
作者：
Kajiya, H;Takekoshi, S;Osamura, R
通讯作者：
Osamura, R
影响因子：
4.1
作者：
Kelly, PA;Bachelot, A;Binart, N
通讯作者：
Binart, N
DOI：
10.1071/bi9800071
发表时间：
1980-03
期刊：
Australian journal of biological sciences
影响因子：
--
作者：
I. Falconer
通讯作者：
I. Falconer
影响因子：
3.2
作者：
Nagai, Y.;Ogasawara, H.;Yamaguchi, T.
通讯作者：
Yamaguchi, T.