Prolonged fasting induces significant germ cell loss in chickens after hatching.
Prolonged fasting induces significant germ cell loss in chickens after hatching.
复制标题
DOI：
10.1016/j.psj.2023.102815
复制
发表时间：
2023-08
期刊：
影响因子：
4.4
通讯作者：
Lu, Yangqing
中科院分区：
文献类型：
--
作者：
Chen, Jiawen;Pu, Liping;Niu, Yajing;Tian, Kui;Jia, Xiaoxuan;Zhang, Lang;Lu, Yangqing
生殖细胞丢失是生殖细胞发育过程中的重要生物学事件。雌性生殖细胞的数量决定了母鸡的繁殖性能和产蛋量。各种内在和外在因素都会影响生殖细胞的丢失，如早期生命中生殖细胞巢的破裂和日常饲养中的营养缺乏。在这里，我们研究了禁食对雏鸡生殖细胞数量的影响。结果表明，禁食72 h的雏鸡生殖细胞损失率高于禁食24 h的结果。RNA-seq分析表明，饥饿雏鸡核糖体途径基因表达下调，内质网蛋白质加工的生物学过程受到抑制。此外，禁食72h的雌性雏鸡，卵巢的qPCR显示核糖体相关基因的下调，卵巢的透射电子显微镜图像显示核糖体较少。血生化指标显示，禁食72h可降低肝功能，影响糖代谢、脂代谢和离子代谢产物。综上所述，本研究结果总结了雏鸡早期禁食对生殖细胞库的负面影响，说明应注意对繁殖期进行适当的管理。
Germ cell loss is a crucial biological event during germ cell development. The number of female germ cells determines the reproductive performance and egg production of hens. Various intrinsic and extrinsic factors affect germ cell loss, such as germ cell nest breakdown in early life and nutritional deficiencies during daily husbandry. Here, we examined the effect of fasting on the germ cell number of chicks. The results showed that 72 h fasting resulted in a higher germ cell loss than that by 24 h fasting in chicks. The RNA-seq analysis revealed that the genes of ribosome pathway were down-regulated and the biological processes of protein processing in endoplasmic reticulum were inhibited in starved chicks. Furthermore, in female chicks treated with 72 h fasting, the qPCR of ovaries showed down-regulation of ribosome-related genes, and transmission electron microscopy imaging of ovaries showed fewer ribosomes. The blood biochemical indices indicated that 72 h fasting reduced the liver functions and affected the glucose metabolism, lipid metabolites and ion metabolites. In summary, the present results concluded negative impacts on the germ cell pool by prolonged fasting in the early life of chicks and manifested that adequate management should be cared for fasted time for breeding.
登录
查看更多内容
影响因子：
4.4
作者：
Wong EA;Uni Z
通讯作者：
Uni Z
影响因子：
4.4
作者：
Yerpes M;Llonch P;Manteca X
通讯作者：
Manteca X
影响因子：
1.5
作者：
Park, Christine J.;Shaughnessy, Mattho P.;Cowles, Robert A.
通讯作者：
Cowles, Robert A.
DOI：
10.1007/978-3-030-54462-1_7
发表时间：
2021-01-01
期刊：
AMINO ACIDS IN NUTRITION AND HEALTH: AMINO ACIDS IN THE NUTRITION OF COMPANION, ZOO AND FARM ANIMALS
影响因子：
--
作者：
He, Wenliang;Li, Peng;Wu, Guoyao
通讯作者：
Wu, Guoyao
影响因子：
7.3
作者：
Lafita-Navarro, M. Carmen;Conacci-Sorrell, Maralice
通讯作者：
Conacci-Sorrell, Maralice