Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.
Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.
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基于涡虫新生细胞的免疫检测和部分照射后 BrdU 标记细胞的追踪来分析体内干细胞运动。
DOI：
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发表时间：
2016
影响因子：
--
通讯作者：
Labib Rouhana
中科院分区：
文献类型：
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作者：
Junichi Tasaki;Chihiro Uchiyama;Labib Rouhana
Planarian扁形虫已成为研究干细胞行为和调控的重要系统。这些生物体能够在损伤或截肢后再生身体的任何部分。在真涡虫再生过程中，一个关键的细胞事件是多能干细胞（称为新母细胞）迁移到损伤部位。在这里，我们描述了两种方法来分析迁移的涡虫干细胞的区域，这些已被局部X射线照射消融。第一种方法涉及免疫标记的有丝分裂neoblast，而第二种是基于跟踪干细胞和它们的后代BrdU掺入后。在细胞运动性研究中使用真涡虫适合于鉴定影响体内干细胞迁移的因素，并且适合于RNA干扰或药理学筛选。
Planarian flatworms have become an important system for the study of stem cell behavior and regulation in vivo. These organisms are able to regenerate any part of their body upon damage or amputation. A crucial cellular event in the process of planarian regeneration is the migration of pluripotent stem cells (known as neoblasts) to the site of injury. Here we describe two approaches for analyzing migration of planarian stem cells to an area where these have been ablated by localized X-ray irradiation. The first approach involves immunolabeling of mitotic neoblasts, while the second is based on tracing stem cells and their progeny after BrdU incorporation. The use of planarians in studies of cell motility is suitable for the identification of factors that influence stem cell migration in vivo and is amenable to RNA interference or pharmacological screening.
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DOI：
10.1073/pnas.96.9.5049
发表时间：
1999-04-27
影响因子：
11.1
作者：
Alvarado, AS;Newmark, PA
通讯作者：
Newmark, PA
影响因子：
2.7
作者：
Wenemoser D;Reddien PW
通讯作者：
Reddien PW
影响因子：
8.8
作者：
Lapan SW;Reddien PW
通讯作者：
Reddien PW
影响因子：
2.7
作者：
Forsthoefel DJ;Park AE;Newmark PA
通讯作者：
Newmark PA