Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF.
Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF.
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DOI：
10.1186/gb-2008-9-7-r110
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发表时间：
2008
期刊：
影响因子：
12.3
通讯作者：
Weinstock, George M.
中科院分区：
文献类型：
--
作者：
Bourgogne, Agathe;Garsin, Danielle A.;Qin, Xiang;Singh, Kavindra V.;Sillanpaa, Jouko;Yerrapragada, Shailaja;Ding, Yan;Dugan-Rocha, Shannon;Buhay, Christian;Shen, Hua;Chen, Guan;Williams, Gabrielle;Muzny, Donna;Maadani, Arash;Fox, Kristina A.;Gioia, Jason;Chen, Lei;Shang, Yue;Arias, Cesar A.;Nallapareddy, Sreedhar R.;Zhao, Meng;Prakash, Vittal P.;Chowdhury, Shahreen;Jiang, Huaiyang;Gibbs, Richard A.;Murray, Barbara E.;Highlander, Sarah K.;Weinstock, George M.
对医院病原体粪肠球菌的两个菌株进行比较表明，不同菌株的毒力介质存在差异，且毒力并不依赖于可移动基因元件。
粪肠球菌已成为一种主要的医院病原体。为了探究其多样性，我们对粪肠球菌菌株OG1RF进行了测序，该菌株通常用于分子操作和毒力研究。
发现OG1RF的2739625个碱基对的染色体与唯一公开可用的已测序菌株V583相比，包含约232千碱基对该菌株所特有的序列。在OG1RF中几乎未发现可移动遗传元件。64个差异区域被分为三类。首先，OG1RF携带39个独特区域，包括2个CRISPR位点和一个新的WxL位点。其次，我们发现了9处替换，即V583特有的序列被OG1RF特有的序列所替代。例如，OG1RF的iol操纵子取代了V583中可能的前噬菌体和vanB转座子。最后，我们发现了16个在V583中存在但在OG1RF中缺失的区域，包括假定的致病岛、几个可能的前噬菌体以及cpsCDEFGHIJK荚膜多糖操纵子。在小鼠腹膜炎模型中，OG1RF比V583致死速度更快，但致死频率更低，并且在小鼠尿路感染模型中，OG1RF大大优于V583。
与V583相比，粪肠球菌OG1RF携带许多独特的位点，但几乎完全缺乏可移动遗传元件表明这不是该物种的一个决定性特征。此外，OG1RF在实验模型中的作用表明，不同粪肠球菌菌株之间的毒力介质可能是多样的，并且毒力不依赖于可移动遗传元件的存在。
A comparison of two strains of the hospital pathogen Enterococcus faecalis suggests that mediators of virulence differ between strains and that virulence does not depend on mobile gene elements Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. faecalis strain OG1RF, which is commonly used for molecular manipulation and virulence studies. The 2,739,625 base pair chromosome of OG1RF was found to contain approximately 232 kilobases unique to this strain compared to V583, the only publicly available sequenced strain. Almost no mobile genetic elements were found in OG1RF. The 64 areas of divergence were classified into three categories. First, OG1RF carries 39 unique regions, including 2 CRISPR loci and a new WxL locus. Second, we found nine replacements where a sequence specific to V583 was substituted by a sequence specific to OG1RF. For example, the iol operon of OG1RF replaces a possible prophage and the vanB transposon in V583. Finally, we found 16 regions that were present in V583 but missing from OG1RF, including the proposed pathogenicity island, several probable prophages, and the cpsCDEFGHIJK capsular polysaccharide operon. OG1RF was more rapidly but less frequently lethal than V583 in the mouse peritonitis model and considerably outcompeted V583 in a murine model of urinary tract infections. E. faecalis OG1RF carries a number of unique loci compared to V583, but the almost complete lack of mobile genetic elements demonstrates that this is not a defining feature of the species. Additionally, OG1RF's effects in experimental models suggest that mediators of virulence may be diverse between different E. faecalis strains and that virulence is not dependent on the presence of mobile genetic elements.
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影响因子：
4.1
作者：
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影响因子：
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