Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of time-wise degraded samples.
Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of time-wise degraded samples.
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稳定的RNA标记以鉴定血液和唾液染色，从时间降解样品的整个基因组表达分析中揭示了。
DOI：
10.1007/s00414-007-0182-6
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发表时间：
2008-03
影响因子：
2.1
通讯作者：
Kayser, Manfred
中科院分区：
文献类型：
--
作者：
Zubakov, Dmitry;Hanekamp, Eline;Kokshoorn, Mieke;van IJcken, Wilfred;Kayser, Manfred
血液和唾液等人类体液是犯罪现场发现的生物材料的最常见来源。法医学中可靠的组织鉴定可以揭示犯罪现场重建的重要见解，从而有助于解决犯罪。现有的推定测试的体液鉴定在法医学中，这通常是基于化学发光或蛋白质分析的局限性，预计将克服基于RNA的方法，提供稳定的RNA标记与组织特异性表达模式。为了产生用于可靠鉴定血迹和唾液斑的稳定RNA标记物组，我们（1）使用AffytoU 133 plus 2基因芯片对一系列随时间降解的血迹和唾液斑样品进行全基因组基因表达分析，（2）查阅表达数据库以获得关于组织特异性的额外信息，和（3）通过定量实时聚合酶链反应确认最有希望的候选基因的表达模式，包括额外的法医学相关组织，如精液和阴道分泌物。总的来说，我们确定了9个稳定的mRNA标记物的血液和5个稳定的mRNA标记物的唾液检测显示组织特异性表达信号的污渍年龄高达180天的年龄，更老。虽然所有的标记物都能够区分血液/唾液和精液样本，但由于阴道分泌物的复杂性质以及口腔和阴道粘膜的生物学相似性，它们都不能区分阴道分泌物。我们建议使用这14个稳定的mRNA标记物，在未来的法医实践中鉴定血液和唾液污渍。本文的在线版本（doi：10.1007/s 00414 -007-0182-6）包含补充材料，可供授权用户使用。
Human body fluids such as blood and saliva represent the most common source of biological material found at a crime scene. Reliable tissue identification in forensic science can reveal significant insights into crime scene reconstruction and can thus contribute toward solving crimes. Limitations of existing presumptive tests for body fluid identification in forensics, which are usually based on chemoluminescence or protein analysis, are expected to be overcome by RNA-based methods, provided that stable RNA markers with tissue-specific expression patterns are available. To generate sets of stable RNA markers for reliable identification of blood and saliva stains we (1) performed whole-genome gene expression analyses on a series of time-wise degraded blood and saliva stain samples using the Affymetrix U133 plus2 GeneChip, (2) consulted expression databases to obtain additional information on tissue specificity, and (3) confirmed expression patterns of the most promising candidate genes by quantitative real-time polymerase chain reaction including additional forensically relevant tissues such as semen and vaginal secretion. Overall, we identified nine stable mRNA markers for blood and five stable mRNA markers for saliva detection showing tissue-specific expression signals in stains aged up to 180 days of age, expectedly older. Although, all of the markers were able to differentiate blood/saliva from semen samples, none of them could differentiate vaginal secretion because of the complex nature of vaginal secretion and the biological similarity of buccal and vaginal mucosa. We propose the use of these 14 stable mRNA markers for identification of blood and saliva stains in future forensic practice. The online version of this article (doi:10.1007/s00414-007-0182-6) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Guo, Ji H.;Maltha, Jaap C.;den Hoff, Johannes. W. Von
通讯作者：
den Hoff, Johannes. W. Von
影响因子：
2.1
作者：
Heinrich, Marielle;Matt, Katja;Schmidt, Ulrike
通讯作者：
Schmidt, Ulrike
DOI：
10.1006/bbrc.1998.8252
发表时间：
1998-03-06
影响因子：
3.1
作者：
Ishibashi, K;Kuwahara, M;Sasaki, S
通讯作者：
Sasaki, S
影响因子：
2.2
作者：
Juusola, J;Ballantyne, J
通讯作者：
Ballantyne, J
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J