Supplementation of macroalgae together with non-starch polysaccharide-degrading enzymes in diets enhanced growth performance, innate immune indexes, and disease resistance against Vibrio parahaemolyticus in rabbitfish Siganus canaliculatus
Supplementation of macroalgae together with non-starch polysaccharide-degrading enzymes in diets enhanced growth performance, innate immune indexes, and disease resistance against Vibrio parahaemolyticus in rabbitfish Siganus canaliculatus
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在日粮中添加大型藻类和非淀粉多糖降解酶可增强兔鱼 Siganus canaliculatus 的生长性能、先天免疫指数和对副溶血性弧菌的抗病能力
DOI：
10.1007/s10811-018-1662-4
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发表时间：
2018-10
影响因子：
3.3
通讯作者：
Yuanyou Li
中科院分区：
文献类型：
--
作者：
Dizhi Xie;Xi Li;Cuihong You;Shuqi Wang;Yuanyou Li
大型海藻具有很高的营养价值，是一种很有潜力的水产饲料原料。然而，大型藻类中存在的抗营养因子如非淀粉多糖（NSP）阻碍了它们的应用。本研究旨在探讨在大型藻类饲料中添加外源性非淀粉多糖降解酶是否可以避免长沟篮子鱼的上述缺点。配制了7种等蛋白（32%）和等脂（8%）饲料（D1-D 7），用于为期10周的幼鱼养殖。D2-D4分别添加12%的大型海藻石莼、龙须菜、孔石莼干粉; D5-D 7在D2-D4的基础上添加0.05%的NSP降解酶。结果表明，D5 ~ D 7组的生长性能与D1组相当，显著高于D2 ~ D4组（P< 0.05）。添加大型海藻组（D2 ~ D 7）的血清溶菌酶、超氧化物歧化酶和酸性磷酸酶活性显著高于D1组（P< 0.05）。不同日粮组间鱼肉氨基酸和脂肪酸组成差异不显著（P> 0.05）。在生长试验结束时，通过腹腔注射105 cfu mL− 1和0.01 mL g− 1体重的副溶血性弧菌，对来自每个饮食组的60条鱼进行攻击。D4-D 7组攻毒后第15天的存活率显著高于D1组。结果表明，大型海藻可以作为一种饲料成分的S。当添加0.05%NSP降解酶时，12%的小导管。此外，饲料中添加大型海藻可提高对虾的天然免疫能力。提高了抗病性。副溶血性
Macroalgae with high nutritional value are a potential ingredient for aquafeed. However, the presence of anti-nutritional factors such as non-starch polysaccharides (NSPs) in macroalgae prevents their application. The present study was conducted to investigate whether the supplementation of exogenous NSP-degrading enzymes in macroalgae-containing diets can avoid the above disadvantage in the rabbitfishSiganus canaliculatus. Seven isoproteic (32%) and isolipidic (8%) diets (D1–D7) were prepared for the culture of rabbitfish juveniles lasting 10 weeks. D1 without macroalgae and NSP-degrading enzymes were used as control diet, D2–D4 contained 12% dry powder of macroalgaeUlva prolifera,Gracilaria lemaneiformis, orUlva pertusa, respectively, while D5–D7 were supplemented with 0.05% NSP-degrading enzymes on the base of D2–D4. The results showed that the growth performance of fish-fed D5–D7 displayed as good as those fed D1 and was significantly higher than those fed D2–D4 (P< 0.05). The activities of serum lysozyme, superoxide dismutase, and acid phosphatase were significantly elevated in fish-fed diets with addition of macroalgae (D2–D7) compared with fish-fed D1 (P< 0.05). The amino acid and fatty acid composition of fillet showed no difference among different dietary groups (P> 0.05). By the end of the growth trial, 60 fish from each dietary group were subjected withVibrio parahaemolyticuschallenge by intra-peritoneally injection at 105cfu mL−1and 0.01 mL g−1body mass. The survival at day 15 post-challenge in fish-fed D4–D7 was significantly higher than those fed D1. The results demonstrated that macroalgae can be used as a dietary ingredient forS. canaliculatusat 12% when supplemented with 0.05% NSP-degrading enzymes. Moreover, the addition of macroalgae in diet can improve the innate immune capability ofS. canaliculatusand enhance the disease resistance againstV. parahaemolyticus.
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