Genetic determinants of endophytism in the Arabidopsis root mycobiome.
Genetic determinants of endophytism in the Arabidopsis root mycobiome.
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DOI：
10.1038/s41467-021-27479-y
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发表时间：
2021-12-10
影响因子：
16.6
通讯作者：
Hacquard S
中科院分区：
文献类型：
--
作者：
Mesny F;Miyauchi S;Thiergart T;Pickel B;Atanasova L;Karlsson M;Hüttel B;Barry KW;Haridas S;Chen C;Bauer D;Andreopoulos W;Pangilinan J;LaButti K;Riley R;Lipzen A;Clum A;Drula E;Henrissat B;Kohler A;Grigoriev IV;Martin FM;Hacquard S
The roots of Arabidopsis thaliana host diverse fungal communities that affect plant health and disease states. Here, we sequence the genomes of 41 fungal isolates representative of the A. thaliana root mycobiota for comparative analysis with other 79 plant-associated fungi. Our analyses indicate that root mycobiota members evolved from ancestors with diverse lifestyles and retain large repertoires of plant cell wall-degrading enzymes (PCWDEs) and effector-like small secreted proteins. We identify a set of 84 gene families associated with endophytism, including genes encoding PCWDEs acting on xylan (family GH10) and cellulose (family AA9). Transcripts encoding these enzymes are also part of a conserved transcriptional program activated by phylogenetically-distant mycobiota members upon host contact. Recolonization experiments with individual fungi indicate that strains with detrimental effects in mono-association with the host colonize roots more aggressively than those with beneficial activities, and dominate in natural root samples. Furthermore, we show that the pectin-degrading enzyme family PL1_7 links aggressiveness of endophytic colonization to plant health. Plant roots host diverse fungal communities that affect plant health. Here, Mesny et al. use comparative genomics and transcriptomics of fungal isolates from the Arabidopsis thaliana root mycobiota, together with root colonization assays, to identify genetic determinants of endophytism.
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DOI：
10.1073/pnas.1710455114
发表时间：
2017-10-31
影响因子：
11.1
作者：
Almario J;Jeena G;Wunder J;Langen G;Zuccaro A;Coupland G;Bucher M
通讯作者：
Bucher M
Microdochium bolleyi（一种海滩草的深色有隔真菌内生菌）的基因组序列草案。
DOI：
10.1128/genomea.00270-16
发表时间：
2016-04-28
期刊：
Genome announcements
影响因子：
--
作者：
David AS;Haridas S;LaButti K;Lim J;Lipzen A;Wang M;Barry K;Grigoriev IV;Spatafora JW;May G
通讯作者：
May G
Biopython：用于计算分子生物学和生物信息学的免费 Python 工具。
DOI：
10.1093/bioinformatics/btp163
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Cock PJ;Antao T;Chang JT;Chapman BA;Cox CJ;Dalke A;Friedberg I;Hamelryck T;Kauff F;Wilczynski B;de Hoon MJ
通讯作者：
de Hoon MJ
影响因子：
2.7
作者：
Curran DM;Gilleard JS;Wasmuth JD
通讯作者：
Wasmuth JD
影响因子：
5.1
作者：
Glynou, Kyriaki;Ali, Tahir;Macia-Vicente, Jose G.
通讯作者：
Macia-Vicente, Jose G.