Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes.
Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes.
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DOI：
10.1186/scrt507
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发表时间：
2014-10-20
影响因子：
7.5
通讯作者：
Boheler KR
中科院分区：
文献类型：
--
作者：
Zhu R;Blazeski A;Poon E;Costa KD;Tung L;Boheler KR
人多能干细胞衍生的心肌细胞（hPSC-CM）是用于实验和临床应用的心肌细胞（CM）的最有前途的来源，但它们的使用在很大程度上受到结构和功能上不成熟的表型的限制，所述表型最接近胚胎或胎儿心脏细胞。应用物理刺激通过机械和生物电转导影响hPSC-CM提供了促进更发育成熟的CM的有力策略。在这里，我们总结了与体内心脏成熟和结构发育相关的主要事件。然后，我们回顾了体外衍生的hPSC-CM的发育状态，同时关注积极参与hPSC-CM的结构和功能适应的物理（电和机械）刺激和贡献（代谢和肥大）因素。最后，我们强调未来可能的调查，应该提供一个更好的理解如何物理刺激可能会促进体外发育，并导致机械的见解领域。需要在使用物理刺激促进发育成熟方面取得进展，以克服目前的局限性，并显着推进hPSC-CM用于心脏疾病建模、体外药物筛选、心脏毒性分析和治疗应用的研究。
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are the most promising source of cardiomyocytes (CMs) for experimental and clinical applications, but their use is largely limited by a structurally and functionally immature phenotype that most closely resembles embryonic or fetal heart cells. The application of physical stimuli to influence hPSC-CMs through mechanical and bioelectrical transduction offers a powerful strategy for promoting more developmentally mature CMs. Here we summarize the major events associated with in vivo heart maturation and structural development. We then review the developmental state of in vitro derived hPSC-CMs, while focusing on physical (electrical and mechanical) stimuli and contributory (metabolic and hypertrophic) factors that are actively involved in structural and functional adaptations of hPSC-CMs. Finally, we highlight areas for possible future investigation that should provide a better understanding of how physical stimuli may promote in vitro development and lead to mechanistic insights. Advances in the use of physical stimuli to promote developmental maturation will be required to overcome current limitations and significantly advance research of hPSC-CMs for cardiac disease modeling, in vitro drug screening, cardiotoxicity analysis and therapeutic applications.
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影响因子：
6.7
作者：
Boheler KR
通讯作者：
Boheler KR
影响因子：
23.9
作者：
Burridge, Paul W.;Keller, Gordon;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
64.8
作者：
Chicurel, ME;Singer, RH;Ingber, DE
通讯作者：
Ingber, DE
DOI：
10.1016/j.pbiomolbio.2012.07.012
发表时间：
2012-10
影响因子：
3.8
作者：
Blazeski A;Zhu R;Hunter DW;Weinberg SH;Boheler KR;Zambidis ET;Tung L
通讯作者：
Tung L
DOI：
10.1016/j.pbiomolbio.2012.07.013
发表时间：
2012-10
影响因子：
3.8
作者：
Blazeski A;Zhu R;Hunter DW;Weinberg SH;Zambidis ET;Tung L
通讯作者：
Tung L