Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
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DOI：
10.1371/journal.pone.0084072
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nishida K
中科院分区：
文献类型：
--
作者：
Hagiwara K;Obayashi T;Sakayori N;Yamanishi E;Hayashi R;Osumi N;Nakazawa T;Nishida K
神经脊细胞(NCC)具有卓越的分化能力和更容易从成人组织中获得的细胞，引起了发育生物学和再生医学的科学家的兴趣。已知NCC的分化潜能取决于它们的起始区。在这里，我们通过分析P0-CRE/FLOXED-EGFP小鼠胚胎中NCC的转录谱和球体形成实验，报道了头面部(CNCCs)和躯干(TNCCs)NCC的不同分子特征。我们发现在cNCC中与致癌相关的基因上调，这些基因以前没有报道与NCC相关，这被认为是关于NCC致癌潜力的一个有趣的特征，如黑色素瘤和神经母细胞瘤。WNT信号相关基因在cNCCs中的表达上调，也提示cNCCs可能参与了肿瘤的发生。我们还注意到间质和神经元标记物分别在cNCC和tNCC中的强烈表达。体外球化和分化实验得到了一致的结果。这些结果与以前关于cNCCs和tNCCs微分电位的概念是一致的。因此，我们认为从P0-CRE/FLOXED-EGFP小鼠中分选NCC可能有助于NCC的基础研究和翻译研究。此外，这些在CNCC中上调的新发现的基因将为NC起源的肿瘤、NCC衍生物中的发育障碍以及NCC在再生医学中的潜在应用提供有用的信息。
The outstanding differentiation capacities and easier access from adult tissues, cells derived from neural crest cells (NCCs) have fascinated scientists in developmental biology and regenerative medicine. Differentiation potentials of NCCs are known to depend on their originating regions. Here, we report differential molecular features between craniofacial (cNCCs) and trunk (tNCCs) NCCs by analyzing transcription profiles and sphere forming assays of NCCs from P0-Cre/floxed-EGFP mouse embryos. We identified up-regulation of genes linked to carcinogenesis in cNCCs that were not previously reported to be related to NCCs, which was considered to be, an interesting feature in regard with carcinogenic potentials of NCCs such as melanoma and neuroblastoma. Wnt signal related genes were statistically up-regulated in cNCCs, also suggesting potential involvement of cNCCs in carcinogenesis. We also noticed intense expression of mesenchymal and neuronal markers in cNCCs and tNCCs, respectively. Consistent results were obtained from in vitro sphere-forming and differentiation assays. These results were in accordance with previous notion about differential potentials of cNCCs and tNCCs. We thus propose that sorting NCCs from P0-Cre/floxed-EGFP mice might be useful for the basic and translational research of NCCs. Furthermore, these newly-identified genes up-regulated in cNCC would provide helpful information on NC-originating tumors, developmental disorders in NCC derivatives, and potential applications of NCCs in regenerative medicine.
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影响因子：
5.3
作者：
Kuhlbrodt, K;Herbarth, B;Wegner, M
通讯作者：
Wegner, M
影响因子：
16.2
作者：
Kang, Shin H.;Fukaya, Masahiro;Yang, Jason K.;Rothstein, Jeffrey D.;Bergles, Dwight E.
通讯作者：
Bergles, Dwight E.
DOI：
10.1242/dev.002642
发表时间：
2007-06
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Billon N;Iannarelli P;Monteiro MC;Glavieux-Pardanaud C;Richardson WD;Kessaris N;Dani C;Dupin E
通讯作者：
Dupin E
影响因子：
5.2
作者：
Chung, Kuei-Fang;Sicard, Flavie;Ehrhart-Bornstein, Monika
通讯作者：
Ehrhart-Bornstein, Monika
影响因子：
30.8
作者：
Comino-Mendez, Inaki;Gracia-Aznarez, Francisco J.;Cascon, Alberto
通讯作者：
Cascon, Alberto